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Volume 10, Number 6—June 2004
Research

Bovine Spongiform Encephalopathy Infectivity in Greater Kudu (Tragelaphus strepsiceros)

Andrew A. Cunningham*Comments to Author , James K. Kirkwood*1, Michael Dawson†2, Yvonne I. Spencer†, Robert B. Green†, and Gerald A.H. Wells†
Author affiliations: *Institute of Zoology, Regent’s Park, London, UK; †Veterinary Laboratories Agency, Addlestone, Surrey, UK; Bovine Spongiform Encephalopathy in Greater Kudu

Main Article

Table 2

Bioassay results for greater kudu tissues injected into C57Bl-J6 mice

Kudu identification Tissuea injected Positive mice/totalb Mean survival period post injection (days) ± SDc Survival period range (d)c
A1212
 Rostral cerebrum
 13/20
 595±84
 428–745
Cranial thoracic spinal cord
 19/20d
 557±121
 413–821
Lumbar spinal cord
 15/19
 521±69
 432–634
Spleen
 3/11d
 819±41
 773–851
Retropharyngeal lymph node
 6/11d
 784±77
 691–921
Popliteal lymph node
 0/20d
 N/Ae
 N/A
Visceral lymph nodes (pool)
 14/18
 622±114
 448–860
Submandibular lymph node
 0/20d
 N/A
 N/A
Distal ileum
 11/20
 547±98
 426–718
Lung
 1/14d
 N/A
 746
Kidney
 0/18
 N/A
 N/A
Caruncular endometrium
 0/20d
 N/A
 N/A
Ovary
 0/20
 N/A
 N/A
Mammary gland
 0/20d
 N/A
 N/A
Submandibular salivary gland
 1/17d
 N/A
 599
Conjunctiva
 1/16d
 N/A
 659
Nasal mucosa
 0/20
 N/A
 N/A
Skeletal muscle (biceps brachii + vastus lateralis)
 0/19d
 N/A
 N/A
Skin (flank)
 2/18d
 713±22
 697–728
Feces
 0/11
 N/A
 N/A
Serum
 0/19
 N/A
 N/A
A664
 Spleen (P)
 1/8d
 N/A
 929
Visceral lymph nodes (pool) (P)
 6/15d
 843±113
 649–952
Lung (P)
 0/20
 N/A
 N/A
Kidney (P)
 0/20
 N/A
 N/A
Skeletal muscle (P)
 0/19
 N/A
 N/A
A1221
 Brainstem
 10/18
 634±62
 541–762
Kidney
 0/20
 N/A
 N/A
Skeletal muscle (vastus lateralis) (F)
 0/19
 N/A
 N/A
A1221 + A666 (pool)
 Spleen (pool)
 0/20
 N/A
 N/A
Visceral lymph nodes (pool)
 10/18
 700±108
 455–851
Lung (pool) (F) 0/19 N/A N/A

aTissues not prepared fresh are suffixed: (F), fixed, (P), paraffin wax embedded.
bNumber of mice positive/number of mice surviving when the first mouse was confirmed positive by histopathologic or immunochemical examination. The denominator for negative groups is the number of mice examined.
cSurvival periods of positive mice determined positive either by histopathologic or immunohistochemical examination results.
dMice examined by PrPSc immunohistochemical examinations.
eN/A, not applicable.

Main Article

1Present affiliation is Universities Federation for Animal Welfare, Wheathampstead, Hertfordshire, UK.

2Present affiliation is National Scrapie Plan Administration Centre, Worcester, UK.

Page created: February 22, 2011
Page updated: February 22, 2011
Page reviewed: February 22, 2011
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