Volume 16, Number 7—July 2010
Dispatch
Rickettsia felis–associated Uneruptive Fever, Senegal
Cristina Socolovschi, Oleg Mediannikov, Cheikh Sokhna, Adama Tall, Georges Diatta, Hubert Bassene, Jean-François Trape, and Didier Raoult
Table 1
Target sequences, primers and probes used in a study of Rickettsia felis, rural Senegal, November 2008–July 2009
| Quantitative real-time PCR designation and specificity | Target gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Probe |
|---|---|---|---|---|
| RKND03 system; Rickettsia genus-specific | gltA | GTG-AAT-GAA-AGA-TTA-CAC-TAT-TTA-T | GTA-TCT-TAG-CAA-TCA-TTC-TAA-TAG-C | 6-FAM-CTA-TTA-TGC-TTG-CGG-CTG-TCG-GTT-C-TAMRA |
| 1029 system; Rickettsia genus-specific | Hypothetical protein (RC0338 gene) | GAM-AAA-TGA-ATT-ATA-TAC-GCC-GCA-AA | ATT-ATT-KCC-AAA-TAT-TCG-TCC-TGT-AC | 6-FAM-CTC-AAG-ATA-AGT-ATG-AGT-TAA-ATG-TAA-A-TAMRA |
| Rpr 331 system; specific for typhus group rickettsiae | Glycosyltransferase | TGC-TTC-ATG-GGC-AAT-GTC-TG | TTG-AGC-ATA-AAA-CTG-CCC-TGC-T | 6-FAM-CGC-TGG-ATT-ATC-AAA-AGA-ATT-AGC-ACG-TAMRA |
| Specific for R. felis | Biotin synthase | ATG-TTC-GGG-CTT-CCG-GTA-TG | CCG-ATT-CAG-CAG-GTT-CTT-CAA | 6-FAM- GCT-GCG-GCG-GTA-TTT-TAG-GAA-TGG-G-TAMRA |
| β-actin; specific for human β-actin gene | Human β-actin | CAT-GCC-ATC-CTG-CGT-CTG-GA | CCG-TGG-CCA-TCT-CTT-GCT-CG | 6-FAM-CGG-GAA-ATC-GTG-CGT-GAC-ATT-AAG-TAMRA |
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