Volume 11, Number 10—October 2005
Dispatch
Detecting Biological Warfare Agents
Linan Song*, Soohyoun Ahn*, and David R. Walt*
Table A2
Cross-reactions between 18 probes on the multiplexed array* (Download PDF [93 KB, 1 page])
| Targets (100 nmol/L) |
Probes
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Bacillus anthracis
|
Brucella melitensis
|
Bacillus thuringiensis kurstaki
|
Yersinia pestis
|
Vaccinia virus
|
Francisella tularensis
|
Clostridium botulinum
|
||||||||||||
|
capA
|
pagA
|
SASP-B
|
Sequence between BMEI1658 and BMEI1659
|
Cry1A
|
plasminogen activator
|
Thymidine kinase
|
fopA
|
Random chromosomal DNA fragment
|
||||||||||
| BA1 | BA2 | BA3 | BA4 | BA5 | BA6 | BM1 | BM2 | BTK1 | BTK2 | YP1 | YP2 | VA1 | VA2 | FT1 | FT2 | CB1 | CB2 | |
| BA1 | 808.1 | 173.4 | 13.8 | 14.5 | 18.1 | 9.61 | 17.1 | 9.75 | 21.1 | 21.3 | 22.2 | 27.9 | 31.8 | 29.2 | 30.8 | 27.4 | 30.1 | 27.2 |
| BA2 | 712.3 | 701.2 | 15.1 | 18.1 | 9.57 | –0.28 | 11.9 | –1.54 | 25.8 | 15.6 | 19.8 | 13.4 | 15.2 | 32.5 | 10.5 | 5.2 | 21.6 | 23.1 |
| BA3 | 9.61 | 13.4 | 457.4 | 6.48 | 6.15 | 7.6 | 12.4 | 11.4 | –2.69 | 13.9 | 5.56 | 6.66 | 11.3 | 24.1 | 8.61 | –2.84 | 29.8 | 16.9 |
| BA4 | 11.7 | 20.8 | 26.9 | 693.2 | 26.6 | 8.61 | 20.3 | 25.5 | 31.6 | 14.4 | 5.65 | 9.05 | 19.6 | 21.6 | 17.9 | 31.1 | 19.8 | 25.6 |
| BA5 | 10.9 | 2.4 | 5.7 | 14.2 | 410.3 | 113.6 | 10.7 | 2.2 | 12.6 | 19.8 | 25.7 | 30.1 | –1.6 | 21.7 | 28.2 | 25.8 | 30.1 | 8.78 |
| BA6 | 17.7 | –3.45 | 11.3 | 15.5 | 262.8 | 853.9 | 17.5 | 31.6 | 29.9 | 11.4 | 33.6 | 32.2 | 24.8 | 27.6 | 30.9 | 11.9 | 13.7 | 31.4 |
| BM1 | 7.92 | 3.32 | 23.2 | –3.22 | 18.8 | 11.5 | 723.5 | –4.28 | 32.2 | –4.99 | 20.8 | 25.8 | 18.2 | 17.3 | 29.3 | 27.9 | 25.9 | 14.6 |
| BM2 | 3.08 | 3.84 | 26.8 | 6.78 | 3.96 | 5.67 | 24.1 | 973.3 | 21.3 | 1.11 | 15.9 | 17.6 | 19.9 | 32.8 | 13.9 | 24.4 | 34.4 | 12.9 |
| BTK1 | 14.1 | 6.1 | 30.4 | 6.35 | 15.6 | 9.77 | 17.1 | 6.05 | 1423.1 | 232.8 | 32.4 | 19.2 | 19.7 | 12.5 | 16.6 | 26.3 | 8.95 | 23.1 |
| BTK2 | 27.7 | –0.91 | 3.39 | 6.8 | 8.39 | 4.56 | 10.3 | 9.59 | 425.8 | 1288.6 | 22.1 | 8.16 | 9.8 | 18.9 | 28.2 | 19 | 7.84 | 19.4 |
| YP1 | 12.5 | –0.79 | 28 | 4.56 | 12.5 | 5.11 | 8.88 | 20.6 | 12.9 | 32.1 | 2089 | 588.7 | 23.6 | 24.6 | 179.7 | 25.7 | 15.9 | 29.2 |
| YP2 | 23.4 | 32.1 | 23.6 | 14.2 | –1.58 | 29.3 | 15.4 | 19.1 | 28.1 | 23.8 | 1491.5 | 1784 | 14.5 | 11.3 | 9.7 | 7.05 | 23.9 | 14.6 |
| VA1 | 8.9 | 24.9 | 25.3 | 4.71 | 8.61 | 18.7 | 13.6 | 8.61 | 3.12 | 9.87 | 12.7 | 16.6 | 1536.9 | 1.75 | 25.4 | 23.8 | 17.7 | 11.8 |
| VA2 | 43.1 | 15.9 | 4.59 | 8.94 | 7.18 | 23.2 | 29.4 | 3.25 | 15.4 | 14.7 | 26.7 | 12 | 18.3 | 1703.4 | 23.9 | –0.76 | 26.3 | 29.7 |
| FT1 | 19.8 | 19.7 | 8.77 | 25.6 | 14.6 | 4.65 | 32.1 | 14.7 | 6.96 | 1.56 | 14.9 | 0.65 | 0.56 | 0.78 | 793 | 26.8 | 28.5 | 20.5 |
| FT2 | 25.4 | 8.4 | 11.5 | 16.9 | 19.7 | 7.98 | 9.94 | 17.9 | 12.5 | 25.1 | 4.44 | 14.5 | 8.3 | 13.4 | 216.7 | 456.9 | 8.99 | 27.9 |
| CB1 | 23.1 | 5.68 | 16.9 | 20.1 | 27.2 | 15.3 | 14.1 | 9.11 | 7.46 | 16.1 | 4.6 | 19 | 28.3 | 171.6 | 4.93 | 24.8 | 843.1 | 18.5 |
| CB2 | 14.8 | 11.2 | 8.45 | 8.16 | 5.45 | 24.6 | 20.9 | 3.25 | 1.62 | 21.9 | 25.8 | 23.5 | 13.3 | 18.0 | 28.2 | 21.2 | 89.1 | 592.1 |
*The numbers are net fluorescence intensities (in arbitrary units) obtained by subtracting the average intensity value of the background from that of the hybridization signal. The specific response for each probe is indicated in red. The cross responses between probes designed for the same biological warfare agent (BWA) are indicated in pink, and the cross-reactions between probes from different BWAs are marked with blue. The standard deviation (SD) of background is 15 (n = 3), and the detection limit of array hybridization for each microsphere probe is 45, defined as 3 × SD.
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