Volume 11, Number 2—February 2005
Dispatch
Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens
Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*
Table 1
Sensitivity of pathogen detection by Mass Tag polymerase chain reaction determined by using plasmid and synthetic RNA standards*
| Pathogen or protein | Detection threshold (DNA copies/RNA copies) |
|---|---|
| Influenza A matrix | 100/1,000 |
| Influenza A N1 | 100/NA |
| Influenza A N2 | 100/NA |
| Influenza A H1 | 100/NA |
| Influenza A H2 | 100/NA |
| Influenza A H3 | 100/NA |
| Influenza A H5 | 100/NA |
| Influenza B H | 500/1,000 |
| RSV group A | 100/1,000 |
| RSV group B | 100/500 |
| Metapneumovirus | 100/1,000 |
| CoV-SARS | 100/500 |
| CoV-OC43 | 100/500 |
| CoV-229E | 100/500 |
| HPIV-1 | 100/1,000 |
| HPIV-2 | 100/1,000 |
| HPIV-3 | 100/500 |
| Chlamydia pneumoniae | 100/NA |
| Mycoplasma pneumoniae | 100/NA |
| Legionella pneumophila | 100/NA |
| Enterovirus (genus) | 500/1,000 |
| Adenovirus (genus) | 5,000/NA |
*NA, not assessed; RSV, respiratory syncytial virus; CoV, coronavirus; SARS, severe acute respiratory syndrome; HPIV, human parainfluenza virus.
1These authors contributed equally to this study.
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