Volume 12, Number 11—November 2006
Research
Clostridium difficile PCR Ribotypes in Calves, Canada
Alexander Rodriguez-Palacios*
, Henry R. Stämpfli*, Todd Duffield*, Andrew S. Peregrine*, Lise A. Trotz-Williams*, Luis G. Arroyo*, Jon S. Brazier†, and J. Scott Weese*
, Henry R. Stämpfli*, Todd Duffield*, Andrew S. Peregrine*, Lise A. Trotz-Williams*, Luis G. Arroyo*, Jon S. Brazier†, and J. Scott Weese*
Table 2
Clostridium difficile PCR ribotypes and toxin genes of 31 isolates obtained from dairy calves in southern Ontario, Canada, 2004
| Toxigenic classification† | Calf PCR ribotypes* | Subtotal, no. (%) | |||||||
| A-11 | B-89 | C-129 | D-189 | E-257 | F-12 | H-75 | I-157 | ||
| A–B–, cdtB– | – | – | – | – | – | 1 | – | – | 1 (3.2) |
| A–B–, cdtB+ | – | – | – | – | – | 2 | – | – | 2 (6.5) |
| A+B+, cdtB– | – | – | 2 | – | 4 | – | 1 | 1 | 8 (25.8) |
| A+B+, cdtB+ | 7 | – | – | 4 | – | – | – | – | 11 (35.5) |
| A–B+, cdtB– | – | 8 | – | – | – | – | – | – | 8 (25.8) |
| A–B+, cdtB+ | – | 1 | – | – | – | – | – | – | 1 (3.2) |
| Subtotal, no. (%) | 7 (22.6) | 9 (29) | 2 (6.5) | 4 (12.9) | 4 (12.9) | 3 (9.7) | 1 (3.2) | 1 (3.2) | 31 (100) |
| Type of tcdC deletion‡, (no.) | A (7) | None (9) | None (2) | B (4) | None (4) | A (3) | None (1) | None (1) | |
| Human PCR ribotypes§ | 078 | 017 | 077 | 027 | 014 | 033 | 078 | NS¶ | |
| *By PCR typing method of Bidet et al. (22). †A, toxic gene tcdA; B, tcdB; – absence or presence of the categorized gene; cdtB, gene that codifies CDTb, the binding segment of the binary toxin. ‡None, tcdC with no deletions (≈345 bp); A, type A deletion of ≈39 bp; B, nondifferentiated type B or C deletion of ≈18 bp (11). §Selected isolates processed at the Anaerobe Reference Laboratory, University Hospital of Wales, Cardiff, UK (23). ¶NS, not submitted for analysis. | |||||||||
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