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Volume 12, Number 11—November 2006
Letter

Real-time PCR for Francisella tularensis Types A and B

Kiersten J. Kugeler*, Ryan Pappert*, Yan Zhou*, and Jeannine M. Petersen*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table A1

Specificity evaluation with DNA from Francisella spp

Organism Sample ID Source Geographic origin Type A assay
(Ct value)* Type B assay
(Ct value)*
F. tularensis subsp.
tularensis (type A)
SchuS4 Human Ohio 15.9 –
WY963418 Human Wyoming 15.9 –
MA002987 Human Massachusetts 18.4 –
CO012364 Cat Colorado 18.3 –
CO013713 Rabbit Colorado 16.5 –
ND000952 Human North Dakota 16.9 –
KS000948 Cat Kansas 16.7 –
OK002731 Human Oklahoma 18.4 –
NC993990 Rabbit North Carolina 16.8 –
AR000028 Human Arkansas 18.8 –
UT983134 Human Utah 16.9 –
NM990295 Rabbit New Mexico 17.2 –
NC973057 Rabbit North Carolina 16.5 –
NC015379 Cat North Carolina 18.6 –
AR982146 Rabbit Arkansas 18.0 –
OK004337 Human Oklahoma 18.4 –
AR011117 Human Arkansas 22.9 –
MO011907 Human Missouri 20.3 –
CA020099
Human
California
18.2
–
F. tularensis subsp.
holarctica (type B)
LVS Rat Russia – 16.0
KY993387 Human Kentucky – 19.7
OR960246 Monkey Oregon – 16.7
CN985979 Human Canada – 17.1
AZ001325 Rat Arizona – 16.2
IL003633 Human Illinois – 18.4
MO011673 Human Missouri – 16.8
KY001708 Human Kentucky – 15.8
OH013029 Prairie dog Ohio – 16.7
UT002098 Human Missouri – 18.2
SP986120 Rabbit Spain – 17.5
IN983055 Rat Indiana – 15.6
CO961243 Vole Colorado – 15.5
CA990837 Human California – 16.5
IN002758 Human Indiana – 15.8
AZ001324 Squirrel Arizona – 15.9
CA993992 Monkey California – 16.9
SP982108 Human Spain – 17.8
NM002642 Human New Mexico – 17.9
JAP5-3-11 Human Japan – 18.6
KO971026 Human Korea – 19.0
F. tularensis subsp.
novicida
GA993548 Human Louisiana – –
GA993549 Human California – –
GA993550 Water Utah – –
UT014992 Human Utah – –
AS020814 Human Australia – –
FX1 Human Texas – –
FX2
Human
Texas
–
–
F. philomiragia
GA012793 Human California – –
GA012794 Human Colorado – –
GA012795 Human New York – –
GA012796 Human California – –
GA012797 Human Pennsylvania – –
GA012799 Human Connecticut – –
GA012800 Human Connecticut – –
GA012801 Human New York – –
GA012802 Human California – –
GA012803 Human New Mexico – –
GA012804 Human Virginia – –
GA012806 Human Massachusetts – –
GA012810 Water Utah – –
GA012811 Water Utah – –
ATCC 25015
Muskrat
Utah
–
–
Francisella-like
tick endosymbionts†
2040372 Tick California – –
2040460 Tick California – –
MV2
Tick
Massachusetts
–
–
Francisella-like
bacteria‡
005 Soil Texas – –
013 Soil Texas – –
015 Soil Texas – –
027 Soil Texas – –
045 Soil Texas – –
034 Soil Texas – –
039 Soil Texas – –

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

Main Article

References
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Main Article

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