Volume 14, Number 7—July 2008
Dispatch
Experimental Infection of Cattle with Highly Pathogenic Avian Influenza Virus (H5N1)
Table 1
Calf | Day postinoculation |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
0 |
1 |
2 |
3–7 |
||||||||
PCR† | PCR | Egg | Cell | PCR | Egg | Cell | PCR | ||||
Inoculated | |||||||||||
A1 | >40 | 20–30 | – | – | 30–40 | – | – | >40 | |||
A2 | >40 | 30–40 | + | + | 30–40 | + | – | >40 | |||
A3 | >40 | 20–30 | + | + | >40 | ND | ND | >40 | |||
A4 |
>40 |
30–40 |
+ |
– |
>40 |
ND |
ND |
>40 |
|||
Contact | |||||||||||
K1 | >40 | >40 | ND | ND | >40 | ND | ND | >40 | |||
K2 | >40 | >40 | ND | ND | >40 | ND | ND | >40 |
*Inoculated calves received highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (8,9); contact calves were not inoculated but were housed with the inoculated calves. Egg, embryonated chicken eggs; cell, MDCK cell culture; +, virus detected; –, virus not detected; ND, not done.
†H5-specific real-time reverse transcription–PCR; results are shown as range of detected threshold cycle values.
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