Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 12—December 2011
Research

Transmission of Guanarito and Pirital Viruses among Wild Rodents, Venezuela

Mary L. Milazzo, Maria N.B. Cajimat, Gloria Duno, Freddy Duno, Antonio Utrera, and Charles F. FulhorstComments to Author 
Author affiliations: University of Texas Medical Branch, Galveston, Texas, USA (M.L. Milazzo, M.N.B. Cajimat, C.F. Fulhorst); Ministerio de Sanidad y Asistencia Social, Region Sanitaria del Estado Portuguesa, Guanare, Venezuela (G. Duno, F. Duno); Universidad Nacional Experimental de Los Llanos Occidentales “Ezequiel Zamora,” Guanare (A. Utrera)

Main Article

Table 1

Strains of Guanarito virus and Pirital virus isolated from the spleens of rodents captured on Hato Maporal and included in the analysis of nucleocapsid protein gene sequences, municipality ofGuanarito, Portuguesa State, Venezuela, 1994–1997

Virus and strain* Rodent no.† Species Date captured Trap site GenBank
accession no.
Guanarito
VAV-623 FHV-623 Zygodontomys brevicauda 1994 Mar 23 JF412365
VAV-952 FHV-952 Z. brevicauda 1994 Jun 6 JF412366
AV 97020997 FHV-4030 Z. brevicauda 1997 Feb 4 A JF412369
AV 97021004 FHV-4037 Z. brevicauda 1997 Feb 4 A JF412370
AV 97021033 FHV-4066 Z. brevicauda 1997 Feb 5 A JF412371
AV 97021034 FHV-4067 Z. brevicauda 1997 Feb 5 A JF412372
AV 97021073 FHV-4106 Z. brevicauda 1997 Feb 11 B JF412373
AV 97021084 FHV-4117 Z. brevicauda 1997 Feb 11 B JF412374
AV 97021092 FHV-4125 Z. brevicauda 1997 Feb 11 B JF412375
AV 97021104 FHV-4137 Z. brevicauda 1997 Feb 12 B JF412376
AV 97021106 FHV-4139 Z. brevicauda 1997 Feb 12 B JF412377
AV 97021113 FHV-4146 Z. brevicauda 1997 Feb 12 B JF412378
AV 97021116 FHV-4149 Sigmodon alstoni 1997 Feb 12 C JF412379
AV 97021117 FHV-4150 Z. brevicauda 1997 Feb 12 C JF412380
AV 97021119 FHV-4152 Z. brevicauda 1997 Feb 12 C AY573922
Pirital
VAV-628 FHV-628 S. alstoni 1994 Mar 23 JF412367
VAV-956 FHV-956 Z. brevicauda 1994 Jun 19 JF412368
AV 97021016 FHV-4049 S. alstoni 1997 Feb 4 B AY573923
AV 97021026 FHV-4059 S. alstoni 1997 Feb 4 B JF412381
AV 97021027 FHV-4060 S. alstoni 1997 Feb 4 B JF412382
AV 97021028 FHV-4061 S. alstoni 1997 Feb 4 B JF412383
AV 97021029 FHV-4062 S. alstoni 1997 Feb 4 B JF412384
AV 97021030 FHV-4063 S. alstoni 1997 Feb 5 A JF412385
AV 97021036 FHV-4069 S. alstoni 1997 Feb 5 A JF412386
AV 97021040 FHV-4073 S. alstoni 1997 Feb 5 A JF412387
AV 97021112 FHV-4145 S. alstoni 1997 Feb 12 B JF412388
AV 97021120 FHV-4153 S. alstoni 1997 Feb 12 C JF412389

*Strains VAV-623, VAV-628, VAV-952, VAV-956, AV 97021016, and AV 97021119 were published previously (3,5). The nucleotide sequences of the 21 other strains were determined in this study. –,rodents were captured in 1994 before establishment of trap sites.
†FHV, Fiebre Hemorrágica Venezolana.

Main Article

References
  1. Salas  R, de Manzione  N, Tesh  RB, Rico-Hesse  R, Shope  RE, Betancourt  A, Venezuelan haemorrhagic fever. Lancet. 1991;338:10336. DOIPubMedGoogle Scholar
  2. Fulhorst  CF, Bowen  MD, Salas  RA, de Manzione  NMC, Duno  G, Utrera  A, Isolation and characterization of Pirital virus, a newly discovered South American arenavirus. Am J Trop Med Hyg. 1997;56:54853.PubMedGoogle Scholar
  3. Fulhorst  CF, Cajimat  MNB, Milazzo  ML, Paredes  H, de Manzione  NMC, Salas  RA, Genetic diversity between and within the arenaviral species indigenous to western Venezuela. Virology. 2008;378:20513. DOIPubMedGoogle Scholar
  4. Musser  GG, Carleton  MD. Family Cricetidae. In: Wilson DE, Reeder DM, editors. Mammal species of the world: a taxonomic and geographic reference. 3rd ed. Baltimore: The Johns Hopkins University Press; 2005. p. 955–1189.
  5. Fulhorst  CF, Bowen  MD, Salas  RA, Duno  G, Utrera  A, Ksiazek  TG, Natural rodent host associations of Guanarito and Pirital viruses (family Arenaviridae) in central Venezuela. Am J Trop Med Hyg. 1999;61:32530.PubMedGoogle Scholar
  6. Milazzo  ML, Duno  G, Utrera  A, Richter  MH, Duno  F, de Manzione  N, Natural host relationships of hantaviruses native to western Venezuela. Vector Borne Zoonotic Dis. 2010;10:60511. DOIPubMedGoogle Scholar
  7. Tesh  RB, Jahrling  PB, Salas  RA, Shope  RE. Description of Guanarito virus (Arenaviridae: Arenavirus), the etiologic agent of Venezuelan hemorrhagic fever. Am J Trop Med Hyg. 1994;50:4529.PubMedGoogle Scholar
  8. Cajimat  MNB, Milazzo  ML, Hess  BD, Rood  MP, Fulhorst  CF. Principal host relationships and evolutionary history of the North American arenaviruses. Virology. 2007;367:23543. DOIPubMedGoogle Scholar
  9. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software, version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMedGoogle Scholar
  10. Felsenstein  J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:78391. DOIGoogle Scholar
  11. Weaver  SC, Salas  RA, de Manzione  N, Fulhorst  CF, Duno  G, Utrera  A, Guanarito virus (Arenaviridae) isolates from endemic and outlying localities in Venezuela: sequence comparisons among and within strains isolated from Venezuelan hemorrhagic fever patients and rodents. Virology. 2000;266:18995. DOIPubMedGoogle Scholar
  12. Weaver  SC, Salas  RA, de Manzione  N, Fulhorst  CF, Travasos da Rosa  AP, Duno  G, Extreme genetic diversity among Pirital virus (Arenaviridae) isolates from western Venezuela. Virology. 2001;285:1108. DOIPubMedGoogle Scholar
  13. Fulhorst  CF, Ksiazek  TG, Peters  CJ, Tesh  RB. Experimental infection of the cane mouse Zygodontomys brevicauda (family Muridae) with Guanarito virus (Arenaviridae), the etiologic agent of Venezuelan hemorrhagic fever. J Infect Dis. 1999;180:9669. DOIPubMedGoogle Scholar
  14. Childs  JE, Peters  CJ. Ecology and epidemiology of arenaviruses and their hosts. In: Salvato MS, editor. The Arenaviridae. New York: Plenum Press; 1993. p. 331–84.

Main Article

Page created: November 30, 2011
Page updated: November 30, 2011
Page reviewed: November 30, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external