Volume 20, Number 4—April 2014
Dispatch
Complete Genome of Hepatitis E Virus from Laboratory Ferrets
Tian-Cheng Li
, Tingting Yang, Yasushi Ami, Yuriko Suzaki, Masayuki Shirakura, Noriko Kishida, Hideki Asanuma, Naokazu Takeda, and Wakita Takaji
, Tingting Yang, Yasushi Ami, Yuriko Suzaki, Masayuki Shirakura, Noriko Kishida, Hideki Asanuma, Naokazu Takeda, and Wakita Takaji
Table 1
Oligonucleotides used to amplify ferret hepatitis E viruses
| Primer (nucleotide positions)* | Sequence, 5′→3′ | Product length, bp† |
|---|---|---|
| Forward FF1 (1–21) | GGCAGACCCCTAATGGAGACA | |
| Reverse FR628 (628–648) | GTTGCGTGCGACATAGGCCTT | 626 |
| Forward FF541 (541–561) | AGCAATGTATCGCCATGGCAT | |
| Reverse FR1535 (1535–1554) | ATCTGCATCAGTCGGGCACA | 1,014 |
| Forward FF1518 (1518–1538) | AGGATCTGACAGTAGACCTGT | |
| Reverse FR2555 (2555–2577) | TGCAATGCCAAATTAGCTGTGT | 1,060 |
| Forward FF2401 (2401–2421) | GGCGATGAGTTGTACCTGTTA | |
| Reverse FR3424 (3432–3445) | GAGCAGCCGGTAACATACTCAA | 1,045 |
| Forward FF3336 (3336–3355) | GCACAATTTCTATCTCACCA | |
| Reverse FR4210 (4210–4230) | ACTCCGAATCAGATGATACA | 985 |
| Forward FF4181 (4181–4202) | GGCTGGTGCACCTGAATGGCT | |
| Reverse FR5800 (5800–5821) | TCAGGCAGACGGCGTATCTTAT | 1,641 |
| Forward FF4812 (4812–4831) | ATGGAGCATGTGTACAAGAT | |
| Reverse TX30SXN | GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | ≈2,050 |
| Reverse FR451‡ | ACACCGTGTGAATCCCTCCGT | |
| Abridged amplification | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | |
| Reverse FR279 (279–300) | ATAGATCTAGGATGCGCACCAA | § |
| Abridged universal amplification | GGCCACGCGTCGACTAGTAC | |
| Reverse FR191 (191–211) | CGGATGCGACCAAACAACAGA | ≈240 |
*Values in parentheses indicate positions of the primer corresponding to the entire genome of hepatitis E virus (JN998607) isolated from ferret.
†Blank cell indicates that 1 primer pair produced 1 product.
‡Used only for cDNA synthesis.
§PCR product was not detected.
Page created: March 18, 2014
Page updated: March 18, 2014
Page reviewed: March 18, 2014
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