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Volume 20, Number 6—June 2014
Research

Genetic Evidence of Importation of Drug-Resistant Plasmodium falciparum to Guatemala from the Democratic Republic of the Congo

Jaymin C. PatelComments to Author , Steve M. Taylor, Patricia C. Juliao, Christian M. Parobek, Mark Janko, Luis Demetrio Gonzalez, Lucia Ortiz, Norma Padilla, Antoinette K. Tshefu, Michael Emch, Venkatachalam Udhayakumar, Kim Lindblade, and Steven R. Meshnick
Author affiliations: University of North Carolina, Chapel Hill, North Carolina, USA (J.C. Patel, S.M. Taylor, C.M. Parobek, M. Janko, M. Emch, S.R. Meshnick); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (P.C. Juliao, V. Udhayakumar, K. Lindblade); Military Medical Center, Guatemala City, Guatemala (L.D. Gonzalez); Universidad de Valle de Guatemala, Guatemala City (L. Ortiz, N. Padilla); University of Kinshasa, Kinshasa, Democratic Republic of the Congo (A.K. Tshefu)

Main Article

Table

Neutral microsatellite loci and primer sequences used for PCR amplification of Plasmodium falciparum malaria genes to identify origin of drug-resistant genotype, Guatemala

Locus name
Chromosome
Primer
Primer sequence (5′→3′)
Tag
Expected product size, bp
C2M33 2 Forward CATTGCAAAATATATATTCTCC FAM 193
Reverse GTGATTTGTACAATGTACATA


Heminested
ATTGCGTAAATAACACATCTGCA


C3M88* 3 Forward CAAAAATGAAAAATGAAAAGG HEX 150
Reverse TAAAGGGTGCGCATATCAAT


Heminested
GTTATTCAAAAAGGACGAAACAAG


C3M69 3 Forward AATAGGAACAAATCATATTG HEX 173
Reverse AGATATCCAGGTAATAAAAAG


Heminested
TTTATGAACACCCTCATGTCACT


C2M29 2 Forward GTGAATAACGGAAAAGGATA FAM 141
Reverse AAGATCAAATACCAGGTGA


Heminested
TTAAGAAACAATCAGAAGCGATG


C2M27 2 Forward CTTTTAATCACTACCATGTTG HEX 117
Reverse ATAATTTAATTGAGGATACCT


Heminested
TTGTATGTATCACTTTTTCATTAC


C3M40 3 Forward GGGTAAAGAAAAACACACAAA FAM 128
Reverse AATGTGTATATTACTAGAAGC


Heminested
TCCGAATATGGAATGTCGAAAG


C3M39 3 Forward CAAGAAGATAGGGATGATAAC FAM 159
Reverse TATTAATTGGTCTTCACCCG


Heminested
GGAGGAACGTAAAGAAGATATTG


C2M34 2 Forward TCCCTTTTAAAATAGAAGAAA FAM 260
Reverse GATTATATGAAAGGATACATG
Heminested TTCACTTTGTAAATTAGAACATATC

*Censored locus caused by poor amplification success.

Main Article

Page created: May 16, 2014
Page updated: May 16, 2014
Page reviewed: May 16, 2014
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