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Volume 21, Number 1—January 2015
Dispatch

Rapid Diagnostic Tests for Identifying Avian Influenza A(H7N9) Virus in Clinical Samples

Yu Chen1, Dayan Wang1, Shufa Zheng, Junhua Li, Wenxiang Chen, Dawei Cui, Jinming Li, Weizhong Yang, Yu Wang, Lanjuan Li2, and Hong Shang2Comments to Author 
Author affiliations: Zhejiang University, Hangzhou, China (Y. Chen, S. Zheng, D. Cui, L. Li); Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou (Y. Chen, S. Zheng, D. Cui, L. Li); National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China (D. Wang, Y. Shu); Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou (D. Wang, Y. Shu); Beijing Hospital Institute of Geriatrics and National Center for Clinical Laboratories, Beijing (W. Chen, J. Li); Chinese Center for Disease Control and Prevention, Beijing (H. Yu, Y. Wang); The First Hospital of China Medical University, Shenyang, China (H. Shang)

Main Article

Table 1

Sensitivity of 6 RDTs for influenza A(H5N7) virus*

RDT Test time, min Storage temperature, °C Shelf life, mo Detection method Type of test Detection limit, TCID50/mL†
Wantai Flu A Dot-ELISA‡ 20–30 2–8 5 Dot-ELISA Well, cartridge 103
Wondfo Flu A§ 15 4–30 8 Colloidal gold Well, cartridge 103
Wondfo H7 Subtype¶ 15 4–30 8 Colloidal gold Well, cartridge 103
BinaxNOW Flu A&B# 15 4–30 24 Colloidal gold Test strip on card 104
ClearView Flu A&B** 15 4–30 24 Colloidal gold Test strip 104
Kaibili Flu A&B†† 15 4–30 18 Colloidal gold Well, cartridge ND

*ND, not detected at the highest tested viral concentration (1 × 107 TCID50/mL); RDT, rapid diagnostic test; TCID50, 50% tissue culture infectious dose. Manufacturer information available at URL for each test.
†Detection limit of A/Anhui/1/2013 (H7N9) virus.
‡http://www.ystwt.cn/flu.html.
§http://www.wondfo.com.cn/English/products/List.aspx?MenuID = 050402&ID = 124&temp = 4.
¶Antigen detection kit for human infection with the H7 subtype avian influenza virus, Wondfo Biological Co., Ltd., Guangzhou, China: approved by China Food and Drug Administration after study.
#http://www.cdc.gov/mmwr/preview/mmwrhtml/mm6132a4.htm.
**http://www.alere.com/ww/en/product-details/clearview-exact-influenza-a-and-b.html.
††http://www.jzmc.cn/content.asp?id=559.

Main Article

1These authors contributed equally to this article.

2These authors contributed equally to this article.

Page created: December 19, 2014
Page updated: December 19, 2014
Page reviewed: December 19, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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