Figure 1

Figure 1. Seroprevalence of Coxiella burnetii (sample size) in wild and farmed European rabbits (Oryctolagus cuniculus), Iberian Peninsula and Chafarinas Islands. The distribution area of wild rabbits in the Iberian Peninsula (10 x...

Serum samples were collected from European wild rabbits in 13 locations in Spain, Portugal, and the Chafarinas Islands during 2003–2013 (Figure 1). Wild rabbits from 1 of the study locations (LO; Figure 1) were obtained from 2 epidemiologic scenarios (10). The first scenario involved rabbits that coexisted with farmed red deer (Cervus elaphus) (sites A and B). The second scenario involved rabbits that had not been in contact with ruminants since 2002 (site C).

In addition to serum samples, spleen, uterus, and mammary gland samples and vaginal and uterus swab specimens were collected from rabbits surveyed at location LO. Each rabbit from this location was weighed and sexed. Serum samples were also collected from farmed rabbits on 4 farms in Spain (Figure 1). Samples were stored at −20°C until tested.

Serum samples were analyzed by using the LSIVet Ruminant Q Fever Serum/Milk ELISA Kit (Life Technologies, Carlsbad, CA, USA) and horseradish peroxidase–conjugated protein G (Sigma-Aldrich, St. Louis, MO, USA) as secondary antibody (10). Results were interpreted according to manufacturer’s recommendations.

DNA from tissues and swab specimens was extracted bu using the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany). Swabs were incubated at 56°C for 30 min in 200 μL of AL buffer containing 20 μL of proteinase K. Swabs specimens were then vortexed for 15 s and removed. The remaining solution was incubated at 56°C for 30 min. The manufacturer’s blood extraction protocol was then used. DNA aliquots were frozen at −20°C. Negative controls (nuclease-free water; Promega, Madison, WI, USA) were included during DNA extraction.

DNA samples were analyzed by using a conventional PCR (11). PCR products were visualized by electrophoresis in 1.2% agarose gels containing 0.1 μL/mL of GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, USA).

Logistic regression models were used to test the effect of potential factors (Table) on the individual risk of exposure to C. burnetii. Individual ELISA results were included as response variables in the models and the location origin of rabbits was used as a random factor.

Logistic regression models were also used for individual exposure of rabbits from location LO to C. burnetii (ELISA), for the presence/absence of C. burnetii DNA in spleen (a proxy of systemic infection), and for the presence/absence of C. burnetii DNA in the reproductive tract (a proxy of shedding; including PCR results from uterus, and vaginal and uterus swab specimens). Location LO was included as a random factor, and sex, weight and ruminant presence/absence were also included as predictor variables (Table). Models were created by using a forward stepwise procedure. The model with the lowest Akaike information criterion (12) was selected.

Statistical analyses were performed in SPSS version 20.0 (IBM, Armonk, NY, USA). Prevalence-associated, Clopper-Pearson exact 95% CIs were estimated.

Serum samples from 572 rabbits (464 wild and 108 farmed) (Figure 1) were analyzed. Overall seroprevalence was 32.3% (95% CI 28.5%–36.4%) for wild and farmed rabbits, 37.9% (95% CI 33.5%–42.5%) for wild rabbits, and 8.3% (95% CI 3.8%–15.2) for farmed rabbits. Seroprevalence in wild rabbit populations ranged from 6.7% to 81.3%. Nine (64.3%) of 13 wild rabbit populations and 2 (50%) of 4 farms had >1 seropositive rabbit. The best model for C. burnetii exposure retained sampling year and season, and the risk for seropositivity was higher in summer (Table).

Figure 2

Figure 2. Prevalence of antibodies against Coxiella burnetii and C. burnetii DNA in European rabbits (Oryctolagus cuniculus) at sampling location LO, Iberian Peninsula. A) Antibodies; B) DNA in spleen; C) DNA in vaginal...

Seroprevalence at location LO was 65.2% (133/204; 95% CI 58.2%–71.7%); it was slightly lower at site C than at sites A and B (Figure 2, panel A). However, none of the considered factors were retained in the best model (Table). Six (4.4%; 95% CI 1.6%–9.4%) of 136 spleen samples analyzed at location LO were positive by PCR (4 male and 2 female rabbits). Five of the 6 spleen PCR–positive animals were seropositive. The 2 female rabbits were positive for C. burnetii DNA in vaginal swab specimens. Spleen PCR–positive rabbits were observed only at sites A and B (Figure 2, panel B).

The best model for the presence of C. burnetii DNA in spleen retained sampling year, season, presence of ruminants, and sex (Table). Results suggest expected higher systemic infection prevalence in rabbits coexisting with farmed red deer (Figure 2, panel B). C. burnetii DNA was detected in the reproductive tract of 9 (14.1%; 95% CI 6.6%–25.0%) of 64 female rabbits at sites A, B, and C (Figure 2, panel F). The presence of ruminants was retained in the best model for C. burnetii DNA in the reproductive tract (Table). None of the 13 mammary glands analyzed was positive for C. burnetii DNA.

This study provides 3 results that suggest that European rabbits might be reservoirs of C. burnetii. These 3 results are high seroprevalence of this bacteria; systemic infections; and bacterial shedding in vaginal secretions, which, in other host species, constitutes the main source for environmental contamination and transmission between species (13).

Host density is a major factor in C. burnetii prevalence in livestock (14). The highest seroprevalence values were observed at 2 locations where rabbit populations are managed for hunting purposes, which promotes high densities of rabbits. These findings suggest that rabbit density may be a major factor in the ecology of C. burnetii. In addition, the European rabbit is a gregarious species with a high reproductive rate. This rate favors transmission of C. burnetii from infected to susceptible animals, which is enhanced by replacement of C. burnetii–negative rabbits and can contribute further to spread of this bacterium in the environment.

The higher risk of exposure to C. burnetii observed during the summer might be related to increased indirect interaction with C. burnetii shed by coexisting ruminants, whose main shedding season is late spring–early summer (3). Inclusion of ruminants in the final models for systemic infection and vaginal shedding at location LO might support this hypothesis. However, further analyses, including molecular typing of circulating strains, would be needed to confirm the direction, frequency, and magnitude of interspecies interactions favoring transmission of C. burnetii.

Indirect transmission of C. burnetii between rabbits, humans, livestock, and other wild species may be enhanced in regions with high-density rabbit populations and in regions in which the European rabbit is a major game or farm species. Hunters, game keepers, rabbit farmers, veterinarians, wildlife researchers, livestock producers and livestock might be exposed to C. burnetii from rabbits (6,15). The European rabbit shows a high potential as a reservoir of C. burnetii for infection of livestock and humans in Europe.