Volume 21, Number 9—September 2015
CME ACTIVITY - Synopsis
Mycobacterium abscessus Complex Infections in Humans
Introduction
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Release date: August 13, 2015; Expiration date: August 13, 2016
Learning Objectives
Upon completion of this activity, participants will be able to:
• Describe clinical and nosocomial aspects of M. abscessus infections, based on a literature review
• Compare clinical and treatment aspects of infections with M. abscessus subspecies
• Describe treatment of M. abscessus infections
CME Editor
Claudia Chesley, Technical Writer/Editor, Emerging Infectious Diseases. Disclosure: Claudia Chesley has disclosed no relevant financial relationships.
CME Author
Laurie Barclay, MD, freelance writer and reviewer, Medscape, LLC. Disclosure: Laurie Barclay, MD, has disclosed no relevant financial relationships.
Authors
Disclosures: Meng-Rui Lee, MD; Wang-Huei Sheng, MD, PhD; Chong-Jen Yu, MD, PhD; and Li-Na Lee, PhD, have disclosed no relevant financial relationships. Chien-Ching Hung, MD, PhD, has disclosed the following relevant financial relationships: served as an advisor or consultant for Gilead Sciences; served as a speaker or member of a speakers bureau for Bristol-Myers Squibb; Gilead Sciences; Janssen Pharmaceuticals, Inc.; received grants for clinical research for Janssen Pharmaceuticals, Inc. Po-Ren Hsueh, MD, has disclosed the following relevant financial relationships: served as a speaker or member of a speakers bureau for AstraZeneca; Bayer; Bectin, Dickinson and Co.; Bruker; MSD; Pfizer, Sanofi-Aventis.
Abstract
Mycobacterium abscessus complex comprises a group of rapidly growing, multidrug-resistant, nontuberculous mycobacteria that are responsible for a wide spectrum of skin and soft tissue diseases, central nervous system infections, bacteremia, and ocular and other infections. M. abscessus complex is differentiated into 3 subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. The 2 major subspecies, M. abscessus subsp. abscessus and M. abscessus subsp. massiliense, have different erm(41) gene patterns. This gene provides intrinsic resistance to macrolides, so the different patterns lead to different treatment outcomes. M. abscessus complex outbreaks associated with cosmetic procedures and nosocomial transmissions are not uncommon. Clarithromycin, amikacin, and cefoxitin are the current antimicrobial drugs of choice for treatment. However, new treatment regimens are urgently needed, as are rapid and inexpensive identification methods and measures to contain nosocomial transmission and outbreaks.
Mycobacteria are divided into 2 major groups for the purpose of diagnosis and treatment: Mycobacterium tuberculosis complex, which comprises M. tuberculosis, and nontuberculous mycobacteria (NTM), which comprise all of the other mycobacteria species that do not cause tuberculosis. NTM can cause pulmonary disease resembling tuberculosis, skin and soft tissue infections (SSTIs), central nervous system infections, bacteremia, and ocular and other infections (1,2). Over the past decade, the number of NTM disease cases worldwide has markedly increased (3,4), and the upsurge cannot be explained solely by increased awareness among physicians and advances in laboratory methods (3).
M. abscessus complex is a group of rapidly growing, multidrug-resistant NTM species that are ubiquitous in soil and water (1). Species comprising M. avium complex (MAC) are the most common NTM species responsible for disease; however, infections caused by M. abscessus complex are more difficult to treat because of antimicrobial drug resistance (5). M. abscessus complex is also resistant to disinfectants and, therefore, can cause postsurgical and postprocedural infections (2,5). Although M. abscessus complex most commonly causes SSTIs and pulmonary infections, the complex can also cause disease in almost all human organs (2,5). To improve our understanding of M. abscessus complex infections, we reviewed the epidemiology and clinical features of and treatment and prevention measure for diseases caused by the organisms as well as the taxonomy and antimicrobial susceptibilities of these organisms.
We performed a PubMed search for M. abscessus complex articles published during January 1990–December 2014, using the following search terms: M. abscessus, M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, M. abscessus subsp. massiliense, M. massiliense, M. bolletii, and nontuberculous mycobacteria. Only articles published with abstracts in English were selected.
Bacterial Classification
Figure 1
Figure 1. Serial changes in the nomenclature and taxonomic classification of Mycobacterium abscessus complex, 1992–2013.
M. abscessus was first isolated from a knee abscess in 1952 (1). M. abscessus and M. chelonae were originally considered to belong to the same species (“M. chelonei” or “M. chelonae”), but in 1992, M. abscessus was reclassified as an individual species (1). After M. abscessus was recognized as an independent species, new subspecies, including M. massiliense and M. bolletii, were discovered. Debate has ensued over whether M. massiliense and M. bolletii should be reunited to form one subspecies, M. abscessus subsp. bolletii (6). It is hoped that the debate will be settled as a result of findings from several recent studies that clearly demonstrated, by genome comparison, that M. abscessus complex comprises 3 entities: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii (7–11). Serial changes in the taxonomic classification and nomenclature of M. abscessus complex, from 1992 to 2013, are shown in Figure 1.
M. abscessus subsp. bolletii is recognized as a rare pathogen with a functional inducible erythromycin ribosome methyltransferase (erm) (41) gene. In most M. abscessus subsp. abscessus mycobacterium, this gene leads to macrolide resistance. M. abscessus subsp. massiliense has been proposed to have a nonfunctional erm(41) gene, leading to macrolide susceptibility and a favorable treatment outcome for infections (7–11).
Laboratory Identification
Figure 2
Figure 2. Spectrum of Mycobacterium abscessus subsp. abscessus and M. abscessus subsp. massiliense created by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Biotyper system (Microflex LT; Bruker Daltonik GmbH, Bremen, Germany). The absolute intensities...
Definitive diagnosis of M. abscessus complex infection in humans is invariably determined by the isolation of M. abscessus complex from clinical specimens. The correct subspecies identification of M. abscessus complex has traditionally relied on phenotypic methods (e.g., biochemical testing for the utilization of citrate) to distinguish them from closely related species like M. chelonae (1). However, this method is not accurate enough to differentiate between the 2 main subspecies of the complex. Instead, rpoB gene–based sequencing is a more reliable method for correctly identifying M. abscessus complex to the subspecies level (10). However, because of the limited differences between the subspecies of M. abscessus complex, some researchers have questioned the accuracy of identification results from the sequencing of a single gene, especially the rpoB gene (10). Many schemes have been used in an attempt to accurately differentiate between subspecies, such as multilocus gene sequence typing, sequencing of the erm gene, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Figure 2) (10,12). Nonetheless, because the taxonomic classification is still changing, the debate over the optimal identification method will probably also continue.
The global isolation and epidemiology of M. abscessus complex are diverse. Furthermore, due to limitations in correct and detailed species identification, previous epidemiologic studies often referred to M. abscessus complex as M. chelonae/abscessus group or rapidly growing mycobacteria (13). In the United States, M. abscessus/chelonae complex infections are secondary only to MAC infections, compromising 2.6%–13.0% of all mycobacterial pulmonary infections across various study sites. This percentage correlates to an annual prevalence of <1 M. abscessus/chelonae pulmonary infections per 100,000 population, but the prevalence is increasing (13). M. abscessus complex is especially prevalent in East Asia. For example, in Taiwan, M. abscessus complex comprises 17.2% of all clinical NTM isolates, which correlates to 1.7 cases/100,000 population (4). According to current studies, the proportion of M. abscessus subsp. massiliense and M. abscessus subsp. abscessus is about the same among all clinical isolates (12). M. abscessus subsp. bolletii is rarely isolated (7).
Respiratory Tract Infections
Figure 3
Figure 3. Chest radiograph (A) and computed tomography scan (B) images for a patient with pulmonary disease due to Mycobacterium abscessus subsp. abscessus. A) The arrow indicates a cavity with surrounding consolidation over...
M. abscessus complex can cause pulmonary disease, especially in vulnerable hosts with underlying structural lung disease, such as cystic fibrosis, bronchiectasis, and prior tuberculosis (2). M. abscessus complex pulmonary disease usually follows an indolent, but progressive, course, causing persistent symptoms, decline of pulmonary function, and impaired quality of life; however, the disease can also follow a fulminant course with acute respiratory failure (2,14). Establishing a diagnosis of pulmonary disease due to M. abscessus complex is not straightforward because isolation of M. abscessus complex from respiratory samples is not, in and of itself, diagnostic of pulmonary disease (2). According to guidelines published by the American Thoracic Society/Infectious Diseases Society of America in 2007, the diagnosis of M. abscessus complex pulmonary disease requires the fulfillment of clinical and microbiological criteria, such as the presence of clinical symptoms; radiographic evidence of lesions compatible with NTM pulmonary disease; appropriate exclusion of other diseases; and, in most circumstances, positive culture results from at least 2 separate expectorated sputum samples (2). Common radiographic findings of M. abscessus complex pulmonary infection (i.e., bronchiolitis; bronchiectasis; nodules; consolidation; and, less frequently, cavities) are shown in Figure 3 (2).
M. abscessus complex is especially prevalent in respiratory specimens from patients with cystic fibrosis (7,15). Recent studies have shown that M. abscessus complex infection is no longer a contraindication for lung transplantation, although postoperative complications and a prolonged treatment course can be expected (7).
Pulmonary disease caused by M. abscessus complex is notoriously difficult to treat. Although there is no standard treatment, current guidelines suggest the administration of macrolide-based therapy in combination with intravenously administered antimicrobial agents; however, this regimen has been shown to have a substantial cytotoxic effect (2). Of 65 patients with pulmonary disease due to M. abscessus complex who received an initial 4-week course of intravenous antimicrobial agents followed by macrolide-based combination therapy, 38 (58%) had M. abscessus–negative sputum samples >12 months after treatment (16). Surgical resection of localized disease in addition to antimicrobial therapy has been shown to elicit a longer microbiologic response than antimicrobial agents alone: sputum samples were M. abscessus complex–negative for at least 1 year in 57% versus 28% of these treatment groups, respectively (17). According to the 2007 American Thoracic Society/Infectious Diseases Society of America guidelines, the treatment options remain limited with current antimicrobial agents, and M. abscessus complex pulmonary disease is still considered a chronic incurable disease (2).
The advancement of subspecies differentiation has allowed for more effective management of pulmonary disease caused by M. abscessus complex. For example, unlike M. abscessus subsp. abscessus, M. abscessus subsp. massiliense does not have inducible resistance to clarithromycin (7). Therefore, knowing that a patient’s infection is due to M. abscessus subsp. massiliense rather than 1 of the other 2 subspecies enables the physician to confidently administer clarithromycin (7,18). In a large study on treatment outcome in patients with pulmonary disease caused by M. Abscessus subsp. massiliense or M. abscessus subsp. abscessus, all patients had similar clinical signs, radiographic findings, and treatment regimens (18). However, after treatment, the percentage of patients who had negative sputum culture results was much higher in the M. abscessus subsp. massiliense–infected group (88%) than in the M. abscessus subsp. abscessus–infected group (25%) (18). The lack of efficacy of clarithromycin-containing antimicrobial therapy against M. abscessus subsp. abscessus isolates in the study could be explained by the subspecies’ inducible resistance to clarithromycin. The study clearly demonstrated how M. abscessus subsp. massiliense and M. abscessus subsp. abscessus have different susceptibility profiles to combination therapy containing clarithromycin and different outcomes from such treatment (18).
SSTIs
Figure 4
Figure 4. Skin lesions caused by Mycobacterium abscessus subsp. abscessus. A) Diffuse erythematous papular eruptions on the face and bilateral cervical lymphadenitis in a middle-aged man. B) A circumscribed subcutaneous nodule with pus...
SSTIs are also commonly caused by M. abscessus complex; infections range from deep tissue infections to localized skin infections. The 2 major mechanisms for acquiring an M. abscessus complex–associated SSTI are by 1) direct contact with contaminated material or water through traumatic injury, surgical wound, or environmental exposure and 2) secondary involvement of skin and soft tissue during disseminated disease (19). SSTIs caused by M. abscessus complex have been reported in patients who recently underwent cosmetic procedures (e.g., mesotherapy), tattooing, and acupuncture (19). M. abscessus complex SSTIs can also develop after exposure to environmental sources, such as spas and hot springs (19,20). More often, however, these SSTIs develop among hospitalized postsurgical patients, in whom surgical wound infections are most commonly due to M. abscessus subsp. massiliense (21,22). Disseminated M. abscessus complex infections with skin and soft tissue involvement also commonly occur (23). Of note, however, the presence of M. abscessus complex SSTIs can result in or from disseminated M. abscessus complex infections (23). M. abscessus complex skin infection have diverse presentations, including cutaneous nodules (usually tender), erythematous papules/pustules, and papular eruptions or abscesses (Figure 4) (19).
Central Nervous System Infections
Figure 5
Figure 5. Brain computed tomography scan images for a patient with central nervous system infection caused by Mycobacterium abscessus subsp. bolletii. Arrows indicate abnormal nodular pachymeningeal thickening and leptomeningeal and intraparenchymal extension with...
Central nervous system (CNS) infections caused by M. abscessus complex are rare, but when they do occur, meningitis and cerebral abscesses are the most common manifestations (Figure 5). Although MAC is responsible for most NTM CNS infections, especially in HIV-infected hosts, M. abscessus complex has increasingly been reported to cause CNS infections in HIV-negative patients (21). In one study, M. abscessus was responsible for most NTM CNS infections in HIV-seronegative patients (8/11 patients), especially in patients who had undergone neurosurgical procedures, patients who had intracranial catheters, and patients with otologic diseases. Treatment outcome depended on the patient’s underlying disease and health status. Clarithromycin-based combination therapy for at least 1 year plus surgical intervention, if needed, offered the best chance for cure (21).
Disseminated Diseases and Bacteremia
Disseminated M. abscessus complex infections, such as lymphadenopathy, SSTIs, pulmonary infections, and bacteremia, are on the rise (23), and bacteremia caused by M. abscessus complex is most often associated with catheter use (24,25). A recent study showed that surgical wound infection may be the portal of entry, especially for M. abscessus subsp. massiliense (26). Optimal treatment modalities include removal of intravascular catheters, surgical debridement, and administration of intravenous antimicrobial agents chosen on the basis of drug susceptibility test results.
Disseminated M. abscessus complex infections tend to occur in immunocompromised hosts, including persons with HIV. However, these infections can also occur in HIV-negative patients. Browne et al. (23) recently showed that neutralizing anti–interferon-γ autoantibodies were present in 81% of HIV-negative patients with disseminated NTM-associated infections, and in adults, these antibodies were associated with adult-onset immunodeficiency similar to that seen in advanced HIV infection. This adult-onset immunodeficiency status can lead to disseminated NTM disease that mimics advanced HIV infection (23).
Ocular Infections
The incidence of NTM ocular infections (keratitis, endophthalmitis, scleritis, and other tissues of the ocular area) has increased over the past decade, and the increase has been attributed to the M. chelonae/abscessus group (27). Interpreting the real trend in ocular infections caused by M. abscessus complex is difficult because most studies have not used reliable tests to differentiate between M. abscessus complex and M. chelonae (27).
Initial treatment of M. abscessus complex ocular infections involves the discontinuation of topical corticosteroids, if used. The optimal treatment strategy (topical therapy, systemic antimicrobial agents, and surgical intervention) depends on the site of the ocular infection (28). Topical therapy, particularly topical amikacin and clarithromycin, can be used to treat some M. abscessus complex ocular infections (e.g., conjunctivitis, scleritis, keratitis, endophthalmitis) (28), and systemic antimicrobial agents can be used for all ocular infections (28). Surgical debridement, including removal of infected tissue, should be considered and is necessary for treatment of infections in some patients (28). Treatment outcome varies according to the site of infection, and early recognition of the infection is crucial.
Outbreaks of M. abscessus complex infections in hospital and clinic settings have been reported worldwide (19). Many of the outbreak events occur in clinics conducting cosmetic surgery, liposuction, mesotherapy, or intravenous infusion of cell therapy (29). Proposed sources of transmission include contaminated disinfectants, saline, and surgical instruments as well as contact transmission between patients (19,30,31).
M. abscessus complex transmission involves vulnerable hosts and causes substantial illness and death; thus, concern is also rising regarding outbreaks in centers specializing in lung transplantation and treatment of cystic fibrosis (30). Whole-genome sequencing of outbreak isolates has provided evidence of patient-to-patient transmission of M. abscessus complex; this transmission is most likely indirect rather than direct (30).
M. abscessus complex is notoriously resistant to standard antituberculous agents and most antimicrobial agents (5). The Clinical and Laboratory Standards Institute recommends testing rapidly growing mycobacteria for susceptibility to macrolides (clarithromycin and amikacin), aminoglycosides, fluoroquinolones, imipenem, doxycycline, tigecycline, cefoxitin, cotrimoxazole, and linezolid (32). The recommended drug susceptibility testing method is broth microdilution in cation-adjusted Mueller-Hinton broth supplemented with oleic albumin dextrose catalase (32). Among the agents suggested for M. abscessus complex susceptibility testing, clarithromycin, amikacin, and cefoxitin have the best in vitro antimycobacterial activity (7,32,33).
Recent major studies presenting susceptibility and resistance rates for M. abscessus subsp. massiliense, M. abscessus subsp. abscessus, and M. abscessus complex against 7 antimicrobial agents are summarized in Table 1. Most of the studies are from Asia, and the resistance rate for clarithromycin ranges from 0 to 38%. The resistance rates for cefoxitin (overall 15.1%) and amikacin (overall 7.7%) are also low. Doxycycline, quinolones (including moxifloxacin and ciprofloxacin), and imipenem had high resistance rates. Therefore, local susceptibility data are needed to guide treatment.
Because of its rarity, M. abscessus subsp. bolletii is discussed separately here. These mycobacteria are uniformly resistant to drugs recommended for use against M. abscessus complex. In one study, high MICs of tested antimycobacterial agents were observed, and amikacin probably had the highest activity (i.e., the lowest MIC) (33).
Recent studies have reported on the importance of the erm(41) gene in M. abscessus complex; this gene confers macrolide resistance through methylation of 23S ribosomal RNA (39). The erm(41) gene is present in the M. abscessus complex group but absent in M. chelonae (39). Many strains of M. abscessus subsp. massiliense have a nonfunctional erm(41) gene, and because of this, the rate of clarithromycin susceptibility is higher in M. abscessus subsp. massiliense than in M. abscessus subsp. abscessus (18). The Clinical and Laboratory Standards Institute recommends testing for inducible macrolide resistance because subspecies of M. abscessus complex demonstrate susceptibility to clarithromycin during the first 3–5 days of incubation but demonstrate resistance after an extended duration of incubation (preferably 14 days, according to many experts) (39).
Another area of strenuous clinical research involves identifying and developing novel anti–M. abscessus complex agents. One such agent, the glycylcycline tigecycline, has been shown to exhibit good in vitro activity against rapidly growing mycobacteria, especially M. abscessus complex (12,21). However, no prospective trial has been conducted to evaluate the efficacy of tigecycline, and a breakpoint for interpreting tigecycline susceptibility has not been established (32).
Several problems regarding treatment of M. abscessus complex infections in different organs are unsolved. For example, there is a lack of consensus on the optimal antimicrobial agents and combination therapy, optimal treatment duration, and the introduction of novel antimicrobial agents (e.g., tigecycline). Reports describing cases of M. abscessus complex infection are limited, except for those describing pulmonary disease and SSTIs. Thus, treatment recommendations must rely on retrospective case series. A summary of treatment recommendations from previous studies is shown in Table 2. The treatment of serious M. abscessus complex disease usually involves initial combination antimicrobial therapy with a macrolide (clarithromycin 1,000 mg daily or 500 mg twice daily, or azithromycin 250 mg–500 mg daily) plus intravenous agents for at least 2 weeks to several months followed by oral macrolide–based therapy (2). The drugs of choice for initial intravenous administration are amikacin (25 mg/kg 3×/wk) plus cefoxitin (up to 12 g/d given in divided doses) or amikacin (25 mg/kg 3×/wk) plus imipenem (500 mg 2–4×/wk) (2). As previously mentioned, the in vitro MICs of tigecycline are low, and the drug should be considered in treatment regimens.
M. abscessus complex infection can be acquired in the community or in the hospital setting. In the community setting, water supply systems have been postulated to be the source of human infections (7,40). Membrane filtration, hyperchlorination, maintenance of constant pressure gradients, and the utilization of particular pipe materials have been suggested as methods for reducing the presence of NTM in water supply systems (7,40). In the hospital setting, disinfectant failure, contamination of medical devices and water, and indirect transmission between patients are considered to be the source of infections (19,30). In addition, clinics for cosmetic procedures have become sites of frequent outbreaks of M. abscessus complex infections (19,29). It is unclear whether patients with M. abscessus complex disease should be isolated from vulnerable hosts, such as patients with cystic fibrosis.
M. abscessus complex comprises a group of rapidly growing, multidrug-resistant, nontuberculous mycobacteria that are responsible for a wide spectrum of SSTIs and other infections. The complex is differentiated into 3 subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii, which is rarely isolated. The major difference between M. abscessus subsp. massiliense and M. abscessus subsp. abscessus is that the former does not have an intact erm(41) gene and thus does not have inducible macrolide resistance; treatment response may thus be better among patients with infections caused by M. abscessus subsp. massiliense. M. abscessus complex can cause infections involving almost all organs, but the infections generally involve the lungs, skin, and soft tissue. Drugs with the best in vitro activity include clarithromycin, amikacin, cefoxitin, and possibly tigecycline. Treatment regimens vary according to the infection site and usually include macrolide-based combination therapy, including parenteral amikacin plus another parenteral agent (cefoxitin, tigecycline, imipenem, or linezolid), for weeks to months, followed by oral antimicrobial therapy. Evidence of nosocomial transmission and outbreaks of M. abscessus complex is increasing; therefore, strenuous infection control measures should be taken to reduce the possibility of hospital-acquired M. abscessus complex infections.
Because of the complexity of the molecular techniques needed to differentiate between M. abscessus subsp. abscessus and M. abscessus subsp. massiliense, it is difficult for most laboratories to identify the different subspecies. A more rapid and less expensive method for subspecies identification is thus needed for epidemiologic and clinical purposes. In addition, prospective trials comparing different regimens of antimicrobial agents are needed to determine the best treatment options; these studies should include novel agents, such as tigecycline. The effect of implementing isolation protocols for patients with infections due to M. abscessus complex (particularly pulmonary disease) should also be evaluated in future studies.
Dr. Lee is attending physician at the Department of Internal Medicine, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu, Taiwan, and Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. His primary research interests include emerging infections and epidemiologic and clinical research on diseases caused by nontuberculous mycobacteria.
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Earning CME Credit
To obtain credit, you should first read the journal article. After reading the article, you should be able to answer the following, related, multiple-choice questions. To complete the questions (with a minimum 75% passing score) and earn continuing medical education (CME) credit, please go to http://www.medscape.org/journal/eid. Credit cannot be obtained for tests completed on paper, although you may use the worksheet below to keep a record of your answers. You must be a registered user on Medscape.org. If you are not registered on Medscape.org, please click on the “Register” link on the right hand side of the website to register. Only one answer is correct for each question. Once you successfully answer all post-test questions you will be able to view and/or print your certificate. For questions regarding the content of this activity, contact the accredited provider, CME@medscape.net. For technical assistance, contact CME@webmd.net. American Medical Association’s Physician’s Recognition Award (AMA PRA) credits are accepted in the US as evidence of participation in CME activities. For further information on this award, please refer to http://www.ama-assn.org/ama/pub/about-ama/awards/ama-physicians-recognition-award.page. The AMA has determined that physicians not licensed in the US who participate in this CME activity are eligible for AMA PRA Category 1 Credits™. Through agreements that the AMA has made with agencies in some countries, AMA PRA credit may be acceptable as evidence of participation in CME activities. If you are not licensed in the US, please complete the questions online, print the certificate and present it to your national medical association for review.
Article Title:
Mycobacterium abscessus Complex Infections in Humans
CME Questions
1. Your patient is a 22-year-old woman with cystic fibrosis in whom you suspect Mycobacterium abscessus infection. According to the literature review by Meng-Rui Lee and colleagues, which of the following statements about clinical and nosocomial aspects of M. abscessus infections is correct?
A. These infections only involve the lungs, skin, and soft tissue
B. Persons with underlying structural lung disease such as cystic fibrosis, bronchiectasis, and previous tuberculosis are at increased risk for lung infections with M. abscessus
C. Isolation of M. abscessus complex from respiratory samples is, in and of itself, diagnostic of pulmonary disease
D. M. abscessus complex is highly sensitive to disinfectants
2. According to the literature review by Meng-Rui Lee and colleagues, which of the following statements about clinical and treatment aspects of infections with M. abscessus, subspecies is correct?
A. Five subspecies are currently identified, with M. abscessus subspecies bolletii being the most common
B. Subspecies identification among M. abscessus complex is readily available and easy to perform in most laboratories
C. Treatment response is best among patients with infections resulting from M abscessus subspecies abscessus
D. M. abscessus subspecies abscessus and massiliense differ in the pattern of the erm(41) gene, which provides intrinsic resistance to macrolides
3. According to the literature review by Meng-Rui Lee and colleagues, which of the following statements about treatment of M. abscessus infections would most likely be correct?
A. Infections from M. abscessus complex are much easier to treat than those resulting from species comprising M. avium complex
B. Treatment regimens usually involve only oral therapy
C. Clarithromycin, amikacin, and cefoxitin are currently considered to be the drugs of choice
D. Treatment is usually complete in 10 days
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Related Links
Table of Contents – Volume 21, Number 9—September 2015
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Please use the form below to submit correspondence to the authors or contact them at the following address:
Po-Ren Hsueh, Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan
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