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Volume 22, Number 2—February 2016
Research

Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India

Lalan Kumar Arya, Sivakumar R. Rathinam, Prajna Lalitha, Usha R. Kim, Sudeep Ghatani, and Veena Tandon1Comments to Author 
Author affiliations: Aravind Medical Research Foundation, Madurai, India (L.K. Arya, P. Lalitha); Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Madurai (S.R. Rathinam, U.R. Kim); North-Eastern Hill University, Shillong, India (S. Ghatani, V. Tandon)

Main Article

Figure 5

PCR amplification of trematode cercaria DNA obtained from Melanoides tuberculata snails in study of ocular inflammation in children, South India. Gel electrophoresis was performed on 1.5% agarose gel. A) Internal transcribed spacer 2 region; arrow indicates 369-bp amplified DNA product. Lanes 1–3, trematode cercariae DNA; lane 4, negative control; lane 5, 100-bp DNA marker. B) Internal transcribed spacer 2 region; arrow indicates 539-bp–amplified DNA product. Lanes 1–4, trematode cercariae DNA;

Figure 5. PCR amplification of trematode cercaria DNA obtained from Melanoides tuberculata snails in study of ocular inflammation in children, South India. Gel electrophoresis was performed on 1.5% agarose gel. A) Internal transcribed spacer 2 region; arrow indicates 369-bp amplified DNA product. Lanes 1–3, trematode cercariae DNA; lane 4, negative control; lane 5, 100-bp DNA marker. B) Internal transcribed spacer 2 region; arrow indicates 539-bp–amplified DNA product. Lanes 1–4, trematode cercariae DNA; lane 5, negative control; lane 6, 100-bp DNA marker. C) 28S rDNA region; arrow indicates 715-bp amplified DNA product. Lanes 1–4, trematode cercariae DNA; lane 5, negative control; lane 6, 100-bp DNA marker.

Main Article

1Current affiliation: National Academy of Sciences, India, Lucknow, India.

Page created: January 13, 2016
Page updated: January 13, 2016
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