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Volume 22, Number 8—August 2016
Research

Virulence and Evolution of West Nile Virus, Australia, 1960–2012

Natalie A. Prow12, Judith H. Edmonds2, David T. Williams, Yin X. Setoh, Helle Bielefeldt-Ohmann, Willy W. Suen, Jody Hobson-Peters, Andrew F. van den Hurk, Alyssa T. Pyke, Sonja Hall-Mendelin, Judith A. Northill, Cheryl A. Johansen, David Warrilow, Jianning Wang, Peter D. Kirkland, Stephen Doggett, Christy C. Andrade3, Aaron C. Brault, Alexander A. Khromykh4Comments to Author , and Roy A. Hall4Comments to Author 
Author affiliations: The University of Queensland, St. Lucia, Queensland, Australia (N.A. Prow, J.H. Edmonds, Y.X. Setoh, H. Bielefeldt-Ohmann, W.W. Suen, J. Hobson-Peters, A.A. Khromykh, R.A. Hall); CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia (D.T. Williams, J. Wang); The University of Queensland, Gatton, Queensland, Australia (H. Bielefeldt-Ohmann, W.W. Suen); Department of Health, Brisbane, Queensland, Australia (A.F. van den Hurk, A.T. Pyke, S. Hall-Mendelin, J.A. Northill, D. Warrilow); The University of Western Australia, Nedlands, Western Australia, Australia (C.A. Johansen); Elizabeth Macarthur Agriculture Institute, Menangle, New South Wales, Australia (P.D. Kirkland); University of Sydney and Pathology West–ICPMR, Westmead, New South Wales, Australia (S. Doggett); University of California, Davis, California, USA (C.C. Andrade, A.C. Brault); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (C.C. Andrade, A.C. Brault)

Main Article

Table 4

Comparison of amino acid sequences between virulent and attenuated West Nile virus strains*

Gene and amino acid position in polyprotein (corresponding protein)
 NY99
 Virulent strains
Attenuated Kunjin strains
Boort, NSW2011, NSW2012, Gu0631
 KUN1960
 K68967
 K2499
 K6453
 K74015
 Hu6774
 Gu1009
C
86 (86) Lys Lys Arg Lys Lys Lys Lys Lys Lys
114 (114)
 Met
 Ile (Boort), Thr (NSW2011, NSW2012, Gu0631)
Met
 Thr
 Thr
 Thr
 Met
 Thr
 Thr
prM
143 (20) Thr Ala Thr Ala Ala Ala Ala Ala Ala
158 (35) Ile Thr Ile Thr Thr Thr Thr Thr Thr
279 (156)
 Val
 Thr
Ala
 Thr
 Thr
 Thr
 Thr
 Thr
 Thr
E
413 (123) Thr Thr Ala Thr Thr Thr Thr Thr Thr
446 (156) Ser Ser Phe Ser Ser Ser Ser Ser Ser
600 (310) Lys Arg Thr Arg Arg Arg Arg Arg Arg
773 (483) Leu Phe Leu Phe Phe Phe Leu Phe Phe
790 (500)
 His
 His
Tyr
 His
 His
 His
 His
 His
 His
NS1
1081 (290)
 Ser
 Asn
Ser
 Asn
 Asn
 Asn
 Ser
 Asn
 Asn
NS2A
1255 (112) Val Val Ala Val Val Val Val Val Val
1272 (129) Ile Ile Met Ile Ile Ile Ile Ile Ile
1366 (223)
 Ile
 Ile
Val
 Ile
 Ile
 Ile
 Ile
 Ile
 Ile
NS3
1520 (146) Lys Lys Arg Lys Lys Lys Lys Lys Lys
1970 (586)
 Asn
 Ser
Asn
 Ser
 Ser
 Ser
 Asn
 Ser
 Ser
NS4A
2179 (55)
 Ala
 Thr
Ala
 Thr
 Thr
 Thr
 Thr
 Thr
 Ala
NS4B
2296 (23) Val Ile Thr Ile Ile Ile Ile Ile Ile
2324 (51) Val Phe Val Phe Phe Phe Val Phe Phe
2368 (95) Ala Ser Ala Ser Ser Ser Ser Ser Ser
2450 (177) Met Ile Met Ile Ile Ile Ile Ile Ile
2518 (245)
 Ile
 Ile
Val
 Ile
 Ile
 Ile
 Ile
 Ile
 Ile
NS5
2629 (101) Arg Lys Arg Lys Lys Lys Arg Lys Lys
3088 (560) Asp Asn Asp Asn Asn Asn Asp Asn Asn

*C, capsid; E, envelope; NS, nonstructural; prM, premembrane.

Main Article

1Current affiliation: QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia.

2These authors contributed equally to the major technical aspects of the work.

3Current affiliation: Willamette University, Salem, Oregon, USA.

4These authors served as joint senior authors.

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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