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Volume 23, Number 2—February 2017
Research

Highly Pathogenic Influenza A(H5Nx) Viruses with Altered H5 Receptor-Binding Specificity

Hongbo Guo1, Erik de Vries1, Ryan McBride, Jojanneke Dekkers, Wenjie Peng, Kim M. Bouwman, Corwin Nycholat, M. Helene Verheije, James C. Paulson, Frank J.M. van Kuppeveld, and Cornelis A.M. de HaanComments to Author 
Author affiliations: Utrecht University, Utrecht, the Netherlands (H. Guo, E. de Vries, J. Dekkers, K.M. Bouwman, M.H. Verheije, F.J.M. van Kuppeveld, C.A.M. de Haan); The Scripps Research Institute, La Jolla, California, USA (R. McBride, W. Peng, C. Nycholat, J.C. Paulson)

Main Article

Figure 9

Histochemical analysis of binding of influenza A virus H5 proteins to avian tissues. A) Duck intestine and chicken trachea tissues stained with an antibody specific for 3′SLeX (anti-3′SLeX). Tissue sections treated with Vibrio cholerae neuraminidase (VCNA) before immunostaining were used as controls. Scale bars indicate 200 μm in left panel and 50 μm in center and right panels. B, C) Duck intestine and chicken trachea tissues incubated with H5 proteins H5N12.3.4 and H5N8 after precomplexing with

Figure 9. Histochemical analysis of binding of influenza A virus H5 proteins to avian tissues. A) Duck intestine and chicken trachea tissues stained with an antibody specific for 3′SLeX (anti-3′SLeX). Tissue sections treated with Vibrio cholerae neuraminidase (VCNA) before immunostaining were used as controls. Scale bars indicate 200 μm in left panel and 50 μm in center and right panels. B, C) Duck intestine and chicken trachea tissues incubated with H5 proteins H5N12.3.4 and H5N8 after precomplexing with horseradish peroxidase (HRP)−conjugated antibodies. Scale bars indicate 200 μm in left panel and 50 μm in right panel. D) Chicken trachea tissues incubated with HRP-conjugated antibodies against H5N12.3.4 (no hemagglutinin [HA]) were used as a negative control. H5N12.3.4, novel H5N1 virus clade 2.3.4. Scale bar indicates 50 μm.

Main Article

1These authors contributed equally to this article.

Page created: January 17, 2017
Page updated: January 17, 2017
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