Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 23, Number 2—February 2017
Research

Swine Influenza Virus (H1N2) Characterization and Transmission in Ferrets, Chile

Nicolás Bravo-Vasquez1, Erik A. Karlsson1, Pedro Jimenez-Bluhm, Victoria Meliopoulos, Bryan Kaplan, Shauna Marvin, Valerie Cortez, Pamela Freiden, Melinda A. Beck, and Stacey Schultz-Cherry2Comments to Author 
Author affiliations: University of Chile, Santiago, Chile (N. Bravo-Vasquez, C. Hamilton-West); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (E.A. Karlsson, P. Jimenez-Bluhm, V. Meliopoulos, B. Kaplan, S. Marvin, V. Cortez, P. Freiden, S. Schultz-Cherry); University of North Carolina, Chapel Hill, North Carolina, USA (M.A. Beck)

Main Article

Figure 4

Replication of influenza viruses in vitro. MDCK cells (A), primary normal human bronchial epithelial cells (B), or primary swine nasal epithelial cells (C) were grown in an air-liquid interface and infected with the indicated viruses at a multiplicity of infection of 0.01. Cell culture supernatants were collected at 6 hours postinfection (hpi), 24 hpi, 48 hpi, and 72 hpi, for MDCK cells and 6 hpi, 24 hpi, 48 hpi, 72 hpi, and 96 hpi for primary normal human bronchial epithelial cells and primary

Figure 4. Replication of influenza viruses in vitro. MDCK cells (A), primary normal human bronchial epithelial cells (B), or primary swine nasal epithelial cells (C) were grown in an air-liquid interface and infected with the indicated viruses at a multiplicity of infection of 0.01. Cell culture supernatants were collected at 6 hours postinfection (hpi), 24 hpi, 48 hpi, and 72 hpi, for MDCK cells and 6 hpi, 24 hpi, 48 hpi, 72 hpi, and 96 hpi for primary normal human bronchial epithelial cells and primary swine nasal epithelial cells, and viral titers were determined by 50% tissue culture infectious dose (TCID50) analysis. Data are presented as mean titer of 3 replicates ± SEM. *p<0.05 versus sw/Chile virus.

Main Article

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: January 17, 2017
Page updated: January 17, 2017
Page reviewed: January 17, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external