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Volume 23, Number 2—February 2017
Dispatch

Low Circulation of Zika Virus, Cambodia, 2007–2016

Veasna Duong, Sivuth Ong, Rithea Leang, Rekol Huy, Sowath Ly, Ugo Mounier, Teyputita Ou, Saraden In, Borin Peng, Sreymom Ken, Philippe Buchy, Arnaud Tarantola, Paul F. Horwood, and Philippe DussartComments to Author 
Author affiliations: Institut Pasteur, Phnom Penh, Cambodia (V. Duong, S. Ong, S. Ly, U. Mounier, T. Ou, S. In, B. Peng, S. Ken, A. Tarantola, P.F. Horwood, P. Dussart); National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh (R. Leang, R. Huy); GlaxoSmithKline Vaccines Research and Development, Singapore (P. Buchy)

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Figure 2

Geographic distribution of Zika virus in Cambodia. PCR- and IgM-positive cases were from 9 different provinces in north, central and south Cambodia. The 5 Zika virus–positive samples by quantitative real-time reverse transcription (qRT-PCR) in this study were distributed as follows: the 2007 (n = 1) sample was received from Kampong Cham province, and the other cases from 2008 (n = 1), 2009 (n = 2), and 2015 (n = 1) were from the Phnom Penh area (red star). The first case of Zika virus infection

Figure 2. Geographic distribution of Zika virus in Cambodia. PCR- and IgM-positive cases were from 9 different provinces in north, central and south Cambodia. The 5 Zika virus–positive samples by quantitative real-time reverse transcription (qRT-PCR) in this study were distributed as follows: the 2007 (n = 1) sample was received from Kampong Cham province, and the other cases from 2008 (n = 1), 2009 (n = 2), and 2015 (n = 1) were from the Phnom Penh area (red star). The first case of Zika virus infection previously reported in Cambodia by conventional RT-PCR was diagnosed in a patient from Kampong Speu province (9). The 16 additional serum samples found to be positive for Zika virus IgM were, from oldest to the most recent, from Phnom Penh (2007, n = 1; 2008, n = 1; 2010, n = 2); Battambang (2007, n = 1; 2012, n = 1); Takeo (2012, n = 1); Kampong Speu (2012, n = 1); Kampot (2012, n = 1; 2015, n = 1); Kampong Chhnang (2015, n = 1); Banteay Meanchey (2015, n = 1); and Siem Reap (2015, n = 4) provinces. Light gray indicates provinces with IgM-positive cases, dark gray indicates province with PCR-positive cases, and black indicates provinces with PCR-positive and IgM-positive cases. Inset shows location of Cambodia in Southeast Asia.

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References
  1. Simpson  DI. Zika virus infection in man. Trans R Soc Trop Med Hyg. 1964;58:335–8. DOIPubMedGoogle Scholar
  2. Musso  D, Gubler  DJ. Zika Virus. Clin Microbiol Rev. 2016;29:487–524. DOIPubMedGoogle Scholar
  3. Duffy  MR, Chen  TH, Hancock  WT, Powers  AM, Kool  JL, Lanciotti  RS, et al. Zika virus outbreak on Yap Island, Federated States of Micronesia. N Engl J Med. 2009;360:2536–43. DOIPubMedGoogle Scholar
  4. Campos  GS, Bandeira  AC, Sardi  SI. Zika virus outbreak, Bahia, Brazil. Emerg Infect Dis. 2015;21:1885–6. DOIPubMedGoogle Scholar
  5. ProMED. Zika virus (44): Singapore, alert, travel advice [cited 2016 Sept 2]. http://www.promedmail.org, archive no. 20160902.4459546
  6. Cauchemez  S, Besnard  M, Bompard  P, Dub  T, Guillemette-Artur  P, Eyrolle-Guignot  D, et al. Association between Zika virus and microcephaly in French Polynesia, 2013-15: a retrospective study. Lancet. 2016;387:2125–32. DOIPubMedGoogle Scholar
  7. Plourde  AR, Bloch  EM. A literature review of Zika virus. Emerg Infect Dis. 2016;22:1185–92. DOIPubMedGoogle Scholar
  8. Huy  R, Buchy  P, Conan  A, Ngan  C, Ong  S, Ali  R, et al. National dengue surveillance in Cambodia 1980-2008: epidemiological and virological trends and the impact of vector control. Bull World Health Organ. 2010;88:650–7. DOIPubMedGoogle Scholar
  9. Heang  V, Yasuda  CY, Sovann  L, Haddow  AD, Travassos da Rosa  AP, Tesh  RB, et al. Zika virus infection, Cambodia, 2010. Emerg Infect Dis. 2012;18:349–51. DOIPubMedGoogle Scholar
  10. Lanciotti  RS, Kosoy  OL, Laven  JJ, Velez  JO, Lambert  AJ, Johnson  AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008;14:1232–9. DOIPubMedGoogle Scholar
  11. Balm  MND, Lee  CK, Lee  HK, Chiu  L, Koay  ESC, Tang  JW. A diagnostic polymerase chain reaction assay for Zika virus. J Med Virol. 2012;84:1501–5. DOIPubMedGoogle Scholar
  12. Centers for Disease Control and Prevention. Interim guidance for Zika virus testing of urine—United States, 2016. MMWR Morb Mortal Wkly Rep. 2016;65:474. DOIPubMedGoogle Scholar
  13. Andries  AC, Duong  V, Ly  S, Cappelle  J, Kim  KS, Lorn Try  P, et al. Value of routine dengue diagnostic tests in urine and saliva specimens. PLoS Negl Trop Dis. 2015;9:e0004100. DOIPubMedGoogle Scholar
  14. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9. DOIPubMedGoogle Scholar
  15. ProMED. Zika virus (11): Americas, Europe, Asia [cited 2016 Mar 1]. http://www.promedmail.org, archive no. 20160301.4059896.

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