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Volume 24, Number 4—April 2018
Research

Avirulent Bacillus anthracis Strain with Molecular Assay Targets as Surrogate for Irradiation-Inactivated Virulent Spores

Roger D. Plaut, Andrea B. Staab, Mark A. Munson, Joan S. Gebhardt, Christopher P. Klimko, Avery V. Quirk, Christopher K. Cote, Tony L. Buhr, Rebecca D. Rossmaier, Robert C. Bernhards, Courtney E. Love, Kimberly L. Berk, Teresa G. Abshire, David A. Rozak, Linda C. Beck, Scott Stibitz, Bruce G. Goodwin, Michael A. Smith, and Shanmuga SozhamannanComments to Author 
Author affiliations: Food and Drug Administration, Silver Spring, Maryland, USA (R.D. Plaut, S. Stibitz); Naval Surface Warfare Center, Dahlgren, Virginia, USA (A.B. Staab, T.L. Buhr, L.C. Beck); Naval Medical Research Center, Fort Detrick, Maryland, USA (M.A. Munson, J.S. Gebhardt); US Army Medical Research Institute of Infectious Diseases, Fort Detrick (C.P. Klimko, A.V. Quirk, C.K. Cote, T.G. Abshire, D.A. Rozak); US Army Edgewood Chemical and Biological Center, Aberdeen Proving Ground, Maryland, USA (R.D. Rossmaier, R.C. Bernhards, C.E. Love, K.L. Berk); Defense Threat Reduction Agency, Fort Belvoir, Virginia, USA (R.C. Bernhards); Joint Research and Development, Inc., Stafford, Virginia, USA (C.E. Love, L.C. Beck); Defense Biological Product Assurance Office, Frederick, Maryland, USA (B.G. Goodwin, M.A. Smith, S. Sozhamannan); The Tauri Group, Inc., Alexandria, Virginia, USA (S. Sozhamannan)

Main Article

Figure 3

Role of Bacillus anthracis toxin components in lethality of Sterne strain 34F2 in female A/J mice and nonlethality of B. anthracis Sterne derivatives. Groups of mice were infected subcutaneously with B. anthracis spores of Sterne strain BA500 (34F2) or isogenic strains deficient for different toxin genes TKO-BAP417 (Δcya,Δlef,ΔpagA); DKO-BAP482 (Δcya,Δlef); BAP708- construct 4 (Δcya, Δlef, ΔpagA) plus insert. Fifty percent lethal dose equivalents ranged from ≈106 to 165. Based on a 1-sided Fishe

Figure 3. Role of Bacillus anthracis toxin components in lethality of Sterne strain 34F2 in female A/J mice and nonlethality of B. anthracis Sterne derivatives. Groups of mice were infected subcutaneously with B. anthracis spores of Sterne strain BA500 (34F2) or isogenic strains deficient for different toxin genes TKO-BAP417 (Δcya, Δlef, ΔpagA); DKO-BAP482 (Δcya, Δlef); BAP708- construct 4 (Δcya, Δlef, ΔpagA) plus insert. Fifty percent lethal dose equivalents ranged from ≈106 to 165. Based on a 1-sided Fisher exact test, p = 0.0003 for all groups (N = 10) versus the control 34F2 (N = 5) group.

Main Article

Page created: March 19, 2018
Page updated: March 19, 2018
Page reviewed: March 19, 2018
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