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Volume 24, Number 9—September 2018
Dispatch

Fatal Tickborne Phlebovirus Infection in Captive Cheetahs, Japan

Keita MatsunoComments to Author , Noriyuki Nonoue, Ayako Noda, Nodoka Kasajima, Keita Noguchi, Ai Takano, Hiroshi Shimoda, Yasuko Orba, Mieko Muramatsu, Yoshihiro Sakoda, Ayato Takada, Shinji Minami, Yumi Une, Shigeru Morikawa, and Ken Maeda
Author affiliations: Hokkaido University, Sapporo, Japan (K. Matsuno, Y. Sakoda, A. Takada); Hiroshima City Asa Zoological Park, Hiroshima, Japan (N. Nonoue, A. Noda, S. Minami); Hokkaido University Research Center for Zoonosis Control, Sapporo (N. Kasajima, Y. Orba, M. Muramatsu, A. Takada); Yamaguchi University, Yamaguchi, Japan (K. Noguchi, A. Takano, H. Shimoda, K. Maeda); Okayama University of Science, Imabari, Japan (Y. Une); National Institute of Infectious Diseases, Tokyo, Japan (S. Morikawa)

Main Article

Figure 1

Detection of severe fever with thrombocytopenia syndrome virus (SFTSV) in samples from 2 cheetahs, Japan, 2017. A) RNA was extracted from tissues, plasma, and serum and subjected to quantitative reverse transcription PCR (RT-PCR). The amounts of SFTSV RNA were quantified, with a reference, as RNA copies/mg for tissues and RNA copies/mL for plasma and serum. The mean of duplicate results is shown in the graph. a, plasma; b, popliteal lymph node (left); c, serum; d, brain; e, salivary gland; f, sp

Figure 1. Detection of severe fever with thrombocytopenia syndrome virus (SFTSV) in samples from 2 cheetahs, Japan, 2017. A) RNA was extracted from tissues, plasma, and serum and subjected to quantitative reverse transcription PCR (RT-PCR). The amounts of SFTSV RNA were quantified, with a reference, as RNA copies/mg for tissues and RNA copies/mL for plasma and serum. The mean of duplicate results is shown in the graph. a, plasma; b, popliteal lymph node (left); c, serum; d, brain; e, salivary gland; f, spleen; g, mesentric lymph node; h, popliteal lymph node (left); i, popliteal lymph node (right). B) The TCID50 of salivary gland (per mg) and swab specimens (per mL) for cheetah 2 was determined using Huh-7 cells. Virus proteins were detected by an immunofluorescence assay with an anti-SFTSV N monoclonal antibody. a, salivary gland; b, oral swab sample; c, nasal swab sample; d, rectal swab sample. ND, not done; TCID50, 50% tissue culture infectious dose.

Main Article

Page created: August 14, 2018
Page updated: August 14, 2018
Page reviewed: August 14, 2018
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