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Volume 25, Number 4—April 2019
Research

Differences in Neuropathogenesis of Encephalitic California Serogroup Viruses

Alyssa B. Evans, Clayton W. Winkler, and Karin E. PetersonComments to Author 
Author affiliations: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA

Main Article

Figure 4

Viral antigen in brains of adult and aged mice exhibiting neurologic disease after intranasal inoculation of 104 PFU of California serogroup (CSG) viruses in study of neuropathogenesis of encephalitic CSG viruses. We evaluated >4 brains from mice infected with each CSG virus, except INKV (where only 3 brains from mice with neurologic disease were available), for viral immunoreactivity. A, B) Representative images showing distribution of virus (white) and virus merged with Hoechst nuclear stai

Figure 4. Viral antigen in brains of adult and aged mice exhibiting neurologic disease after intranasal inoculation of 104 PFU of California serogroup (CSG) viruses in study of neuropathogenesis. We evaluated >4 brains from mice infected with each CSG virus, except INKV (where only 3 brains from mice with neurologic disease were available), for viral immunoreactivity. A, B) Representative images showing distribution of virus (white) and virus merged with Hoechst nuclear stain (blue) via full-section brain scans. Scale bar indicates 1 mm. C) Maximum intensity projections of 5-μm confocal z-stacks (original magnification ×63) of brains of mice infected with the indicated CSG virus, labeled for virus (white) and the neuronal marker microtubule-associated protein 2 (MAP2; red). Left panels demonstrate MAP2 staining alone, and right panels are overlays of virus, MAP2, and Hoechst nuclear stain (blue). Yellow arrows indicate the soma of neurons where both viral and MAP2 immunoreactivity are found. Scale bar indicates 10 μm. INKV, Inkoo virus; JCV, Jamestown Canyon virus; LACV, La Crosse virus; SSHV, snowshoe hare virus; TAHV, Tahyna virus.

Main Article

Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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