Blastomycosis has been reportable to the Minnesota Department of Health (MDH) since 1985. Beginning in 1999, MDH routinely collected information on demographics, illness history, diagnostic test results, treatment, outcomes, and any exposures by using a standardized case report form. Data collection evolved over time; during 1999–2015, case report forms were completed by providers or their staff, and during 2016–2018, MDH staff abstracted medical records and completed case report forms. During the entire study period, a confirmed case of blastomycosis was defined as illness in a Minnesota resident with any of the following: a positive Blastomyces culture, Blastomyces organisms visualized in tissue or body fluid, or a positive Blastomyces antigen test result and compatible clinical illness (e.g., cough, fever, abnormal pulmonary imaging, or skin lesions). Cases were classified as pulmonary only, nonpulmonary (localized disease outside the pulmonary system with no clinical pulmonary illness), or disseminated (disease in both the pulmonary system and at least 1 other system/site). We collected illness onset date, date of first visit to a healthcare provider, and date of the first test for blastomycosis regardless of test result. To assess diagnostic delays, we defined the patient interval as the time between illness onset and first visit to healthcare and the provider interval as the time between first healthcare visit and sample collection date for the first blastomycosis test (which indicates that a blastomycosis diagnosis was under consideration). Total time to diagnosis was defined as the time from illness onset to the first test for blastomycosis. We used the date of first test regardless of result to evaluate the time until healthcare providers considered a systemic mycotic infection. Doing so eliminated the variability in growth rate of Blastomyces cultures.

We attempted to interview all patients or next of kin regarding patients’ illness and exposure history during the 3 months before illness onset, including home and neighborhood environment, occupation, outdoor activities and travel, concurrent medical conditions, immunosuppressive medications, smoking history, and family members or pets with a blastomycosis diagnosis. Underlying conditions included diabetes mellitus, chronic lung disease (e.g., chronic obstructive pulmonary disease, asthma), chronic liver disease (e.g., cirrhosis, hepatitis), and other chronic illnesses (e.g., HIV infection/AIDS, sarcoidosis, heart disease, kidney disease). Immunosuppressive medications included corticosteroids, tumor necrosis factor–α blockers, chemotherapy, or posttransplant medications. Patients were also asked about any information missing from case report forms regarding demographics, symptoms, and prescribed antibacterial and antifungal drugs. On the basis of exposure information obtained during interviews, we assigned the most likely location of Blastomyces exposure for each patient, either a specific Minnesota county or an out-of-state location. This subjective assessment considered incubation period, travel, and activities.

We included in our analysis confirmed cases with a positive specimen collection date of 1999 through 2018. We did not include patients with positive antigen test results but no compatible illness, positive serologic antibody tests only, or other fungal infections.

We calculated incidence by race by using the number of cases and race population in Minnesota for each year (25) and then averaged the yearly incidence rates. We calculated incidence by county by using the average population of each county for the entire period and the average number of cases in each county. We classified counties with an incidence rate of >3 cases/100,000 population as highly blastomycosis-endemic counties, based on a natural break in the distribution of incidence by county.

We analyzed data by using SAS 9.2 statistical software (https://www.sas.com) and conducted univariate analysis by using χ², Fisher exact, Student t, Wilcoxon rank-sum, and Kruskal-Wallis tests. To control for race and sex in analyses of outcome and concurrent conditions, we used multivariate logistic regression. We considered 2-sided p-values of <0.05 to be significant.

The epidemiology and clinical courses of blastomycosis cases in Minnesota are similar to those in other disease-endemic regions. The case-fatality rate, sex ratio, age distribution, and reported symptoms are consistent with those reported from other disease-endemic areas (3,5,7,8). Although case counts and fatality rates were higher in the most recent 2 years, we found no overall trends by year, race, or sex. The typical blastomycosis patient in Minnesota is a 45-year-old white man who spends time outdoors. However, this report highlights underrecognized features of blastomycosis epidemiology, particularly patients who do not fit the typical presentation or demographic.

Although the blastomycosis incidence rate was highest among American Indian/Alaska Natives, the incidence difference between that group and white, Asian, and black persons was not statistically significant. However, in Minnesota, the population of American Indian/Alaska Natives is much smaller than that of other races, which, combined with other factors such as prevalence rates for concurrent conditions or geographic location of residence, may influence incidence rates in the American Indian/Alaska Native population. For example, a larger proportion of American Indian/Alaska Native blastomycosis patients (70%) than patients of other races (38%) live in highly blastomycosis-endemic counties (OR 3.9, 95% CI 1.9–7.8; p<0.001). Genetics may also play a role. Others have discussed increased susceptibility to disease or severe disease after Blastomyces infection for Asian, black, and American Indian/Alaska Native persons (3,4,19,23,24,26). Our data show higher case-fatality rates for Asians and American Indians/Alaska Natives; however, the difference was not significant, even after controlling for sex and underlying conditions. Another study evaluating mortality rates found that the likelihood of dying from blastomycosis-related complications was higher for American Indian/Alaska Native and black patients than for white patients (23). Variation in blastomycosis incidence and outcomes by race warrants further exploration (26).

Hospitalization and mortality rates were higher among patients with underlying conditions or immunocompromised status, as previously reported (5,7,27). Those patients reported outdoor and soil exposures less frequently than did previously healthy patients, which could lead a clinician to discount blastomycosis as a diagnostic possibility. Because the odds of death are dramatically higher for patients with an underlying condition than for previously healthy patients, earlier consideration of alternative pneumonia etiologies (beyond antimicrobial drug–resistant bacterial infection) for those patients is warranted.

Diagnosis of blastomycosis is often delayed. For half of the patients in this study, >1 full month elapsed between illness onset and the patient’s first test that could diagnose blastomycosis. Diagnosis took even longer for those with nonpulmonary infections. Provider interval accounted for a larger proportion of this time than did patient interval. Medical record abstraction provided ample anecdotal evidence that patients visited healthcare providers numerous times before their first blastomycosis test. These delays have many possible consequences, including unnecessary antibacterial drug use and higher hospitalization rates. Being hospitalized seemed to be a key factor in being tested for blastomycosis; 60% of patients were not tested until hospital admission. Earlier testing may have prevented some of these admissions. Relatively few patients underwent urine or serum antigen testing, which, despite cross-reactivity with other fungal pathogens, may have provided guidance toward a general diagnosis of fungal disease and appropriate treatment.

Time to diagnosis should logically be shorter for patients with skin lesions because visible lesions might trigger sampling or consideration of blastomycosis in a patient with concurrent pneumonia. This result was found for 123 Mississippi patients, for whom clinicians correctly diagnosed 64% of blastomycosis cases for patients with skin lesions on their initial visit but only 18% of blastomycosis cases for all patients (28). However, in our study, we compared patients with skin lesions with patients without skin lesions and found that provider delay and total time to diagnosis were significantly longer for those with skin lesions than without skin lesions (data not shown).

Time to diagnosis was also significantly shorter for patients who died than for those who survived. Both patient interval and provider interval were shorter for patients who died, probably because their blastomycosis was more severe from the onset, which may have resulted in earlier presentation to healthcare and more aggressive initial diagnostics. However, patients who died were not tested for a median of 11 days after their first healthcare visit, and perhaps some might not have died had a diagnosis been reached sooner.

We anticipated that time to diagnosis would be shorter for patients residing in highly blastomycosis-endemic counties because local providers would be more familiar with the disease and would order testing earlier. Although the median time to diagnosis was the same in highly and less disease-endemic counties, patients living in highly disease-endemic counties were 50% less likely to be hospitalized. This finding may indicate that where providers were more familiar with blastomycosis, they more frequently ordered testing before hospitalization was required.

Although incidence by county of residence provides a measure of disease frequency, 29% of patients were probably exposed outside their home county. Mapping of case totals for county of exposure compared with county of residence illustrates that many patients live in more populated, less blastomycosis-endemic counties, such as Hennepin and Ramsey (i.e., the Minneapolis–St. Paul metropolitan area) but are exposed in highly blastomycosis-endemic northern counties (e.g., Cass, Itasca). By tracking suspected exposure locations, we can more accurately distinguish highly blastomycosis-endemic areas. Further refinement of such areas and future exposure location data will provide more information about the ecologic niche of blastomycosis and help focus awareness campaigns among healthcare providers, residents, and visitors. Many of the highly blastomycosis-endemic counties attract seasonal residents and tourists in summer, underscoring the value of travel histories for patients with infectious disease and provider familiarity with geographic areas where risk for Blastomyces exposure is greater.

As previously reported, blastomycosis cases were skewed heavily by patient sex. This finding is often attributed to a perceived higher likelihood of male patients having engaged in recreational and occupational outdoor activities that increase risk for exposure to Blastomyces (1,5). In Minnesota, 2 other outdoor-associated diseases that occur in similar locations, Lyme disease and anaplasmosis, also occur more often in men, but the male:female ratio (60:40) is less dramatic (29). Male blastomycosis patients reported participation in some outdoor activities at significantly higher proportions than did female patients. However, even when evaluating an exposure that should not intuitively differ by sex, such as nearby excavation, 72% of patients were male. Other factors may explain differences by sex, such as hormonal effects, as have been proposed for other diseases (30–32).

A previous retrospective case–control study did not find any association between infection and factors typically associated with blastomycosis in outbreaks, such as proximity to water, recreational activities, or soil-related activities (4). A prospective case–control study would help determine whether those who acquire blastomycosis participate in these exposure activities at higher rates. Most patients in this study reported >1 activity typically considered a risk factor for blastomycosis, but no activities were common to all or most patients. In addition, this study does not enable us to determine which exposures present the highest risk because background exposure rates for Minnesota residents are not readily available.

Study limitations include those characteristic of surveillance data. Because the surveillance system is passive, undercounting is possible if cases were not reported. Race and ethnicity were not always documented in medical records. Data collection methods changed in 2016 when medical record abstraction was added. Back and bone pain were added to case report forms in 2001; joint pain, boating, and ATV use were added in 2010. Data regarding the number of healthcare visits were not consistently available. We used the date of first test for blastomycosis as the endpoint for determining time to diagnosis. However, negative test results are not routinely reported. Although we made every effort to collect this information from medical record abstraction or providers, some could have been missed.

In conclusion, to reduce the time to diagnosis and case-fatality rates for patients with blastomycosis, providers should consider alternative etiologies for patients with pneumonia that is unresponsive to antibacterial drugs. Complete travel and exposure histories should be obtained, and antigen testing should be considered as a screening test. Blastomycosis should be considered an emerging risk for immunocompromised or chronically ill patients in disease-endemic regions, even for those who do not report classic exposures. Public health officials should work to increase awareness among persons who live and visit blastomycosis-endemic areas so they can advocate for themselves.