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Volume 27, Number 9—September 2021
Research

Development and Clinical Evaluation of a CRISPR-Based Diagnostic for Rapid Group B Streptococcus Screening

Lingxiao Jiang1, Weiqi Zeng1, Wanting Wu1, Yingying Deng, Fusheng He, Wenli Liang, Mingyao Huang, Hong Huang, Yongjun Li, Xiaorui Wang, Hang Su, Shilei PanComments to Author , and Teng Xu1Comments to Author 
Author affiliations: Zhujiang Hospital, Southern Medical University, Guangzhou, China (L. Jiang, Y. Deng, W. Liang, M. Huang); Vision Medicals Center for Medical Research, Shenzhen, China (W. Zeng, W. Wu, H. Huang, Y. Li, X. Wang, H. Su, T. Xu); Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China (W. Zeng, T. Xu); Zhujiang Hospital, Southern Medical University, Guangzhou (S. Pan)

Main Article

Figure 2

Analytical assessment of the sensitivity and specificity of CRISPR-based diagnostic for rapid GBS screening. Evaluation was performed by testing contrived negative swab samples with indicated CFUs of GBS (A), different copy numbers of GBS genomic DNA (B), and various microbes as interfering materials (C). GBS, group B Streptococcus. A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; E. faecalis, Enterococcus faecalis; hDNA, human DNA; P.aeruginosa, Pseudomonas aeruginosa; S. aureus, Staphylococcus aureus; S. mitis, Streptococcus mitis; S. pneumoniae, Streptococcus pneumoniae; S. pyogenes, Streptococcus pyogenes.

Figure 2. Analytical assessment of the sensitivity and specificity of CRISPR-based diagnostic for rapid GBS screening. Evaluation was performed by testing contrived negative swab samples with indicated CFUs of GBS (A), different copy numbers of GBS genomic DNA (B), and various microbes as interfering materials (C). GBS, group B Streptococcus. A. baumannii, Acinetobacter baumannii; E. coli, Escherichia coli; E. faecalis, Enterococcus faecalis; hDNA, human DNA; P.aeruginosa, Pseudomonas aeruginosa; S. aureus, Staphylococcus aureus; S. mitis, Streptococcus mitis; S. pneumoniae, Streptococcus pneumoniae; S. pyogenes, Streptococcus pyogenes.

Main Article

1These authors contributed equally to this article.

Page created: July 14, 2021
Page updated: August 25, 2021
Page reviewed: August 25, 2021
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