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Volume 28, Number 12—December 2022
Dispatch

Highly Diverse Arenaviruses in Neotropical Bats, Brazil

Luiz Gustavo Bentim Góes, Carlo Fischer, Angélica Cristine Almeida Campos, Cristiano de Carvalho, Andrés Moreira-Soto, Guilherme Ambar, Adriana Ruckert da Rosa, Debora Cardoso de Oliveira, Wendy Karen Jo, Ariovaldo P. Cruz-Neto, Wagner André Pedro, Luzia Helena Queiroz, Paola Minoprio, Edison L. Durigon, and Jan Felix DrexlerComments to Author 
Author affiliations: Charité-Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany (L.G.B. Góes, C. Fischer, A.C.A. Campos, A. Moreira-Soto; W.K. Jo, J.F. Drexler); Scientific Platform Pasteur-USP, São Paulo, Brazil (L.G.B. Góes, A.C.A. Campos, P. Minoprio); Universidade de São Paulo, São Paulo, Brazil (L.G.B. Góes, A.C.A. Campos, E.L. Durigon); Universidade Estadual Paulista, Araçatuba, Brazil (C. de Carvalho, W.A. Pedro, L.H. Queiroz); Universidade Estadual Paulista, Rio Claro, Brazil (G. Ambar, A.P. Cruz-Neto); Centro de Controle de Zoonooses, São Paulo (A. Ruckert da Rosa, D. Cardoso de Oliveira); German Centre for Infection Research (DZIF), Berlin (W.K. Jo, J.F. Drexler)

Main Article

Figure 2

Phylogenetic analyses of highly diverse arenaviruses in neotropical bats, Brazil. Maximum-likelihood consensus trees compare partial RNA-dependent RNA polymerase genes (A), complete large (L) segment genes (B), and complete small (S) segment genes (C) from arenaviruses detected in Artibeus and Carollia spp. bats. Phylogenetic trees were generated using MEGA X software (https://www.megasoftware.net). Bold indicates sequences obtained from this study. Stars indicate regions where arenavirus-positive bat hosts were detected (Figure 1, panel B). Black dots at tree nodes represent bootstrap values >75% (1,000 replicates). Green lines indicate clade A new world arenaviruses, red lines indicate clade B new world arenaviruses, blue lines indicate clade C new world arenaviruses, and purple lines indicate recombinant new world arenaviruses (tentative clade D) (14). GenBank sequences used for comparisons and virus abbreviations (accession nos.): TCRV_Art_lit_TRI_1956, Tacaribe virus strain TRVL-11573 L segment (MT081317) and S segment (MT081316.1); TCRV_Amb_ame_USA_2012, Tacaribe virus Florida L segment (KF923401) and S segment (KF923400); OCEV, Ocozocoautla de Espinosa Virus S segment (JN897398; L segment not available); JUNV, Junin virus L segment (AY35802) and S segment (NC_005081.1); MACV, Machupo virus L segment (AY358021) and S segment (NC_005078.1); CPVX, Cupixi virus L segment (AY216519) and S segment (NC_010254.1); AMAV, Amapari virus L segment (AY216517) and S segment (NC_010247.1); GTOV, Guanarito virus L segment (AY358024) and S segment (NC_005077.1); SABV, Sabia virus L segment (AY358026) and S segment (NC_006317.1); APOV, Apore virus L segment (MF317491) and S segment (MF317490); CHAPV, Chapare virus L segment (EU260464) and S segment (EU260463.1); XAPV, Xapuri virus L segment (MG976577) and S segment (NC_055439.1), LATV, Latino virus L segment (EU627612) and S segment (NC_010758.1); OLVV, Oliveros virus L segment (AY216514) and S segment (NC_010248.1); BCNV, Bear Canyon virus L segment (AY924390) and S segment (NC_010256.1); WWAV, Whitewater Arroyo virus L segment (AY924395) and S segment (NC_010700.1); TAMV, Tamiami virus L segment (AY924393) and S segment (NC_010701.1); FLEV, Flexal virus L segment (EU627611) and S segment (NC_010757.1); PARV, Parana virus L segment (EU627613) and S segment (NC_010756.1); PIRV, Pirital virus L segment (AY494081) and S segment (NC_005894.1); PICV, Pichinde virus L segment (AF427517) and S segment (MK896487.1); LASV, Lassa virus L segment (U73034) and S segment (NC_004296.1); ALLV, Allpahuayo virus L segment (AY216502) and S segment (NC_010253.1). Origins of arenaviruses are indicated for each sample: ARG, Argentina; BOL, Bolivia; BRA, Brazil; COL, Colombia; PER, Peru; TRI, Trinidad; USA, United States of America; VEN, Venezuela. Scale bars indicate nucleotide substitutions per site.

Figure 2. Phylogenetic analyses of highly diverse arenaviruses in neotropical bats, Brazil. Maximum-likelihood consensus trees compare partial RNA-dependent RNA polymerase genes (A), complete large (L) segment genes (B), and complete small (S) segment genes (C) from arenaviruses detected in Artibeus and Carollia spp. bats. Phylogenetic trees were generated using MEGA X software (https://www.megasoftware.net). Bold indicates sequences obtained from this study. Stars indicate regions where arenavirus-positive bat hosts were detected (Figure 1, panel B). Black dots at tree nodes represent bootstrap values >75% (1,000 replicates). Green lines indicate clade A new world arenaviruses, red lines indicate clade B new world arenaviruses, blue lines indicate clade C new world arenaviruses, and purple lines indicate recombinant new world arenaviruses (tentative clade D) (14). GenBank sequences used for comparisons and virus abbreviations (accession nos.): TCRV_Art_lit_TRI_1956, Tacaribe virus strain TRVL-11573 L segment (MT081317) and S segment (MT081316.1); TCRV_Amb_ame_USA_2012, Tacaribe virus Florida L segment (KF923401) and S segment (KF923400); OCEV, Ocozocoautla de Espinosa Virus S segment (JN897398; L segment not available); JUNV, Junin virus L segment (AY35802) and S segment (NC_005081.1); MACV, Machupo virus L segment (AY358021) and S segment (NC_005078.1); CPVX, Cupixi virus L segment (AY216519) and S segment (NC_010254.1); AMAV, Amapari virus L segment (AY216517) and S segment (NC_010247.1); GTOV, Guanarito virus L segment (AY358024) and S segment (NC_005077.1); SABV, Sabia virus L segment (AY358026) and S segment (NC_006317.1); APOV, Apore virus L segment (MF317491) and S segment (MF317490); CHAPV, Chapare virus L segment (EU260464) and S segment (EU260463.1); XAPV, Xapuri virus L segment (MG976577) and S segment (NC_055439.1), LATV, Latino virus L segment (EU627612) and S segment (NC_010758.1); OLVV, Oliveros virus L segment (AY216514) and S segment (NC_010248.1); BCNV, Bear Canyon virus L segment (AY924390) and S segment (NC_010256.1); WWAV, Whitewater Arroyo virus L segment (AY924395) and S segment (NC_010700.1); TAMV, Tamiami virus L segment (AY924393) and S segment (NC_010701.1); FLEV, Flexal virus L segment (EU627611) and S segment (NC_010757.1); PARV, Parana virus L segment (EU627613) and S segment (NC_010756.1); PIRV, Pirital virus L segment (AY494081) and S segment (NC_005894.1); PICV, Pichinde virus L segment (AF427517) and S segment (MK896487.1); LASV, Lassa virus L segment (U73034) and S segment (NC_004296.1); ALLV, Allpahuayo virus L segment (AY216502) and S segment (NC_010253.1). Origins of arenaviruses are indicated for each sample: ARG, Argentina; BOL, Bolivia; BRA, Brazil; COL, Colombia; PER, Peru; TRI, Trinidad; USA, United States of America; VEN, Venezuela. Scale bars indicate nucleotide substitutions per site.

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Page created: October 07, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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