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Volume 28, Number 3—March 2022
Research

Novel Hendra Virus Variant Detected by Sentinel Surveillance of Horses in Australia

Edward J. Annand1Comments to Author , Bethany A. Horsburgh1, Kai Xu, Peter A. Reid, Ben Poole, Maximillian C. de Kantzow, Nicole Brown, Alison Tweedie, Michelle Michie, John D. Grewar, Anne E. Jackson, Nagendrakumar B. Singanallur, Karren M. Plain, Karan Kim, Mary Tachedjian, Brenda van der Heide, Sandra Crameri, David T. Williams, Cristy Secombe, Eric D. Laing, Spencer Sterling, Lianying Yan, Louise Jackson, Cheryl Jones, Raina K. Plowright, Alison J. Peel, Andrew C. Breed, Ibrahim Diallo, Navneet K. Dhand, Philip N. Britton, Christopher C. Broder, Ina Smith2, and John-Sebastian Eden2
Author affiliations: EquiEpiVet, Equine Veterinary and One Health Epidemiology, Aireys Inlet, Victoria, Australia (E.J. Annand); Department of Agriculture, Water, and the Environment Epidemiology and One Health Section, Canberra (E.J. Annand, M.C. de Kantzow, A.C. Breed); University of Sydney School of Veterinary Science and Institute for Infectious Diseases, Sydney, New South Wales, Australia (E.J. Annand, N. Brown, A. Tweedie, A.E. Jackson, K.M. Plain, N.K. Dhand); CSIRO Health and Biosecurity Black Mountain Laboratories, Canberra, Australian Capital Territory, Australia (E.J. Annand, M. Michie, I. Smith); Westmead Institute for Medical Research, Sydney (B.A. Horsburgh, K. Kim, J.-S. Eden); University of Sydney School of Medicine, Sydney (B.A. Horsburgh, C. Jones, P.N. Britton, J.-S. Eden); Ohio State University College of Veterinary Medicine, Columbus, Ohio, USA (K. Xu); Private equine veterinary practice, Brisbane, Queensland, Australia (P.A. Reid); Cooroora Veterinary Clinic, Cooroy, Queensland, Australia (B. Poole); JData, Cape Town, South Africa (J.D. Grewar); University of Pretoria, Pretoria, South Africa (J.D. Grewar); CSIRO Australian Centre for Disease Preparedness, Geelong, Victoria, Australia (N.B. Singanallur, M. Tachedjian, B. van der Heide, S. Crameri, D.T. Williams); Murdoch University School of Veterinary Medicine and The Animal Hospital, Murdoch, Western Australia, Australia (C. Secombe); Uniformed Services University of the Health Sciences Microbiology and Immunology, Bethesda, Maryland, USA (E.D. Laing, S. Sterling, L. Yan, C.C. Broder); Queensland Department of Agriculture and Fisheries Biosecurity Sciences Laboratory, Brisbane (L. Jackson, I. Diallo); Children’s Hospital at Westmead, Infectious Diseases, Sydney (C. Jones, P.N. Britton); Montana State University, Bozeman, Montana, USA (R.K. Plowright); Griffith University Centre for Planetary Health and Food Security, Brisbane (A.J. Peel); University of Queensland School of Veterinary Science, Gatton, Queensland, Australia (A.C. Breed)

Main Article

Table 1

Oligonucleotides used for duplex quantitative reverse transcription PCR targeting the matrix gene of novel Hendra virus variant from horse in Australia

Virus Name Sequence, 5′ → 3′* Reference
Prototype
 Mr_fwd_1 CTTCGACAAAGACGGAACCAA (34)
Mr_rev_1 CCAGCTCGTCGGACAAAATT
Mr_prb_1
 FAM-TGGCATCTT-ZEN-TCATGCTCCATCTCGG-IABk
Variant Mv_fwd_1 TCTCGACAAGGACGGAGCTAA Referent
Mv_rev_1 CCGGCTCGTCGAACAAAATT
Mv_prb_1 HEX-TGGCATCCT-ZEN-TCATGCTTCACCTTGG-IABk

*FAM and HEX 5′ reporter dyes were combined with ZEN Internal Quencher and the 3′ quencher Iowa Black, and supplied by Integrated DNA Technologies (https://www.idtdna.com).

Main Article

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1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

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