Figure 1

Figure 1. Histology of brain and spinal cord used to detect rustrela virus (RusV) in wild mountain lion (Puma concolor) with staggering disease, Colorado, USA. RusV RNA was detected by...

Prenecropsy radiology revealed no skeletal abnormalities to explain the clinical signs observed. Necropsy results indicated poor body condition and mild bruising at the torso and limbs. The stomach contained only pine needles. Histopathology demonstrated severe nonsuppurative meningoencephalomyelitis (Appendix). The leptomeninges were multifocally and markedly expanded by lymphocytes and histiocytes in both brain and spinal cord (Figure 1). Virchow-Robin perivascular spaces were expanded by dense cuffs of lymphocytic to lymphohistiocytic infiltrates up to 20 cell layers thick in nearly all regions of the brain (Figure 1, panels A–C) and spinal cord. Inflammation was largely restricted to the leptomeninges and gray matter, and only minimal in the white matter (Figure 1, panels A, B). Affected sections also demonstrated scattered neuronal necrosis, gliosis, and loose glial nodules (Figure 1, panels B, E, G), partially leading to an irregular architecture (Figure 1, panel G). The cerebellar cortex showed no indication of inflammation or degenerative process (Figure 1, panel I)

Initial diagnostic tests did not detect feline panleukopenia virus, canine distemper virus, West Nile virus, Toxoplasma gondii, influenza A virus, rabies virus, or feline infectious peritonitis virus in the central nervous system (Appendix Table 1). We used pooled brain and spinal cord tissue to extract total RNA (Appendix), then conducted metatranscriptome sequencing to obtain sequence fragments (reads). We used those fragments to de novo assemble a single contiguous sequence (contig) with homology to known sequences of rustrela virus (RusV). The contig represented the whole viral genome and matched RusV reference strains. We submitted the annotated RusV genome sequence to the International Nucleotide Sequence Database Collaboration (https://www.insdc.org; accession no. PP025855).

We adapted real-time reverse transcription PCR primers and probe for RusV (1) by using degenerate bases for consensus homology to the Colorado mountain lion–derived sequence and European RusV sequences (Appendix Table 2). Those adapted methods showed RusV RNA in a pooled sample of brain and spinal cord from the mountain lion, with a cycle threshold value of 20.3.

RusV (Rubivirus strelense), a member of the family Matonaviridae, was recently identified as the cause of staggering disease (1), a usually fatal neurologic syndrome in cats. Since the 1970s, staggering disease has been documented in domestic cats in Europe, predominantly in Sweden and Austria (2–6). Affected cats show a consistent combination of histologic lesions and clinical signs, including hind limb ataxia or paresis, and nonsuppurative meningoencephalitis restricted to the gray matter but not affecting white matter or the cerebellar cortex (1,4,5). A similar syndrome was reported in cats from Alabama, USA, in 1979 but the etiology remained obscure (7). In Germany, RusV has been detected in a broad range of zoo animals with neurologic disorders, including lions (Panthera leo) (8–11).

Because initial diagnostic tests were negative in this case, and history, histopathology, and metatranscriptome sequencing suggested staggering disease, we sent tissue samples and sequence data from the mountain lion to the Friedrich-Loeffler-Institut (Greifswald-Insel Riems, Germany) for additional analyses. To demonstrate an association between the lesions and the virus, we used previously developed in situ hybridization methods for RusV (1,6) (Appendix), which demonstrated RusV RNA in all regions of the brain and nearly all levels of the spinal cord, irrespective of an inflammatory reaction. Only lumbosacral nerve roots (cauda equina) tested negative. RusV-specific RNA localized in neuronal cell bodies (Figure 1, panels D, F, H, J), disseminated within the neuropil of the gray matter (Figure 1, panel D, inlay) and, to a lesser extent, in the white matter. We found particularly abundant or large, dot-like signals in the granule cell layer of the hippocampus (Figure 1, panel H), and in Purkinje cells of the cerebellum (Figure 1, panel J), similar to findings from staggering disease cases in cats from Europe (4).

Figure 2

Figure 2. Sequence similarity and phylogenetic position of RusV in wild mountain lion (Puma concolor) with staggering disease, Colorado, USA. A) Mean pairwise sequence identity between the novel Colorado RusV...

The overall architecture of the viral genome of the novel RusV from Colorado matched those of known RusV (Appendix Figure 2). The mean pairwise nucleotide identity between the novel RusV sequence and sequences from Germany was 69.9% and between sequences from Austria and Sweden was 68.9%; the sequences from Europe shared 76.7% identity among each other (Figure 2, panel A). The mean pairwise amino acid identities of the nonstructural and structural polyproteins ranged from 75.6% to 78.1% between the novel RusV sequence and the sequences from Europe (Figure 2, panel A). The genetic diversity was not equally distributed over the genome; part of the protease and the intergenic region showed especially high levels of sequence variations (Appendix Figure 2).

We performed phylogenetic analysis to compare the RusV sequence from Colorado with appropriate reference strains using an amino acid alignment of the structural polyprotein (Appendix). Those findings suggested classification of the novel RusV as a member of the family Matonaviridae, genus Rubivirus, placing it basal to the known RusV sequences detected in Germany, Sweden, and Austria (Figure 2, panel B). The basal position of the novel RusV in relation to all other known RusV is also supported by phylogeny based on the whole-genome nucleotide sequence (Appendix Figure 3).

Our results demonstrate the presence of a RusV variant in North America that is divergent from those previously described from Europe. The clinical signs, histologic lesions, and infected target cells observed for the wild mountain lion in Colorado, USA, meet the case definition for staggering disease. A causative role for RusV is likely, further supporting previous work identifying RusV as the causative agent of staggering disease in domestic cats from Austria, Sweden, and Germany (1,6), and in lions from zoologic collections in Germany (10).

This report is limited to a single case of staggering disease in Colorado. To determine whether RusV is enzootic in this region, we recommend further investigations, including retrospective RusV testing of tissues from feline encephalitis cases of unknown causes in North America. Surveillance for RusV in small rodents might identify a local reservoir host because rodents of the genus Apodemus have been identified as likely RusV reservoir hosts in Europe through real-time reverse transcription PCR and sequencing of mice brain tissues (1,8,9,12,13). Although Apodemus mice are not indigenous to North America, several genera of small rodents are found throughout Colorado (14,15) and could serve as candidates for further screenings. In addition, future studies should consider that the zoonotic potential of RusV has not been determined.

Of note, a remarkably broad range of other mammalian RusV hosts has been identified in Germany, including equids, mustelids, rodents, and marsupials (8,9,11,12), raising concerns about a zoonotic potential of RusV (8,9). Given the wide host range of the virus in Europe, RusV should be considered as a possible cause for neurologic diseases in all mammal species in North America.