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Volume 31, Number 3—March 2025
Research

Effect of Prior Influenza A(H1N1)pdm09 Virus Infection on Pathogenesis and Transmission of Human Influenza A(H5N1) Clade 2.3.4.4b Virus in Ferret Model

Xiangjie SunComments to Author , Jessica A. Belser, Zhu-Nan Li, Nicole Brock, Joanna A. Pulit-Penaloza, Troy J. Kieran, Claudia Pappas, Hui Zeng, Jessie C. Chang, Paul J. Carney, Brandon L. Bradley-Ferrell, James Stevens, Terrence M. Tumpey, Min Z. Levine, and Taronna R. Maines
Author affiliation: Author affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Figure 1

Detection of cross-reactive antibodies in study of the effect of prior influenza A(H1N1)pdm09 virus infection on pathogenesis and transmission of human influenza A(H5N1) clade 2.3.4.4b virus in ferret model. After primary pH1N1 infection, we detected cross-reactive antibodies by using a high-throughput multiplex influenza antibody detection assay. Serum samples from naive and preimmune ferrets were prediluted 200-fold and added to plates containing antigen-coated microspheres, then plates were incubated with protein A–phycoerythrin conjugate. Reported values represent the mean of duplicate assays. Antibody titers were expressed as median fluorescence intensity. Full virus strain names and sources of all antigens are provided (Appendix 1 Table). HA, hemagglutinin; IAV, influenza A virus; IBV, influenza B virus; NA, neuraminidase; NP, nucleoprotein.

Figure 1. Detection of cross-reactive antibodies in study of the effect of prior influenza A(H1N1)pdm09 virus infection on pathogenesis and transmission of human influenza A(H5N1) clade 2.3.4.4b virus in ferret model. After primary pH1N1 infection, we detected cross-reactive antibodies by using a high-throughput multiplex influenza antibody detection assay. Serum samples from naive and preimmune ferrets were prediluted 200-fold and added to plates containing antigen-coated microspheres, then plates were incubated with protein A–phycoerythrin conjugate. Reported values represent the mean of duplicate assays. Antibody titers were expressed as median fluorescence intensity. Full virus strain names and sources of all antigens are provided (Appendix 1 Table). HA, hemagglutinin; IAV, influenza A virus; IBV, influenza B virus; NA, neuraminidase; NP, nucleoprotein.

Main Article

Page created: December 30, 2024
Page updated: February 28, 2025
Page reviewed: February 28, 2025
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