Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax
Bioterrorism-related Anthrax
Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States
Alex R. Hoffmaster*
, Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*
, Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*
Table 1
Primers used for amplification and sequencing of the pagA gene
| Primer | Sequence (5´ – 3´) |
|---|---|
| PA1575Fa | CGA ACT GAT ACA CGT ATT TTA G |
| PA4191Ra | AGG ATT ATG ATG ATT TAG ATT ACT |
| PA1566Fa | TTT ATC CGA ACT GAT ACA CGT ATT |
| PA4205Ra | ACA AAC AAT CTC AAA GGA TTA TGA |
| PA-1Fa | ATA TTT ATA AAA GTT CTG TTT AAA AAG CC |
| PA-1Ra | TAA ATC CTG CAG ATA CAC TCC CAC |
| PA-2Fa | ATA AGT AAA AAT ACT TCT ACA AGT AGG ACA C |
| PA-2Ra | GAT TTA GAT TAC TGT TTA AAA CAT ACT CTC C |
| PA-3 | TCA TGT AAC AAT GTG GGT AGA TGA C |
| PA-4 | CTC TAT GAG CCT CCT TAA CTA CTG AC |
| PA-5F | ATC CTA GTG ATC CAT TAG AAA CGA C |
| PA-5R | CTT CTC TAT GAG CCT CCT TAA CTA CTG |
| PA-5Fnest | AGT GAT CCA TTA GAA ACG AC |
| PA-5Rnest | TAA CTA CTG ACT CAT CCG C |
| PA-U2121 | TAC ATT TGC TAC TTC CGC TGA TAA |
| PA-L3892 | TGT TTT TCC ATC TTG CCG TAA |
| 2121R | TTA TCA GCG GAA GTA GCA AAT GTA |
| 3892F | TTA CGG CAA GAT GGA AAA ACA |
| 2557R | AGC CGT GCT CCA TTT TTC AGG |
| 3318R | TGC GGT AAC ACT BTCA CTC CAG |
| 2560F | GAA AAA TGG AGC ACG GCT TCT |
| 2924F | CTT GGG CTG AAA CAA TGG GTT |
aPrimers used for amplification of the pagA gene. All other primers were used for sequencing.
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