Table 1

First Isolation of West Nile virus from a Patient with Encephalitis in the United States

Cinnia Huang*

, Brett Slater*, Robert Rudd*, Nandakishore Parchuri†, Rene Hull*, Michelle Dupuis*, and Alexander Hindenburg†

Author affiliations: *Wadsworth Center, New York State Department of Health, Albany, New York, USA; †Winthrop University Hospital, Mineola, New York, USA;

Main Article

Oligonucleotide primers and probe used in the standard RT-PCR and TaqMan assays^a

Primer	Genome target	Genome position^b	Sequence (5′–3′)	RT-PCR product size (bp)
CU9093	NS₅	9097–9120	AGYMGRGCHATHTGGTWYATGTGG	206
CL9279	NS₅	9302–9283	TTCCAVCCDGCKGTRTCATC

D87F	NS₅	10034–10051	GCTCCGCTGTCCCTGTGA	70
D156R	NS₅	10103–10083	CACTCTCCTCCTGCATGGATG

Forwardddd	ENV	1160–1180	TCAGCGATCTCTCCACCAAAG	70
Reverse	ENV	1229–1209	GGGTCAGCACGTTTGTCATTG
Probe	ENV	1186–1207	TGCCCGACCATGGGAGAAGCTC

^aRT-PCR, reverse transcription–polymerase chain reaction.
^bGenome position according to GenBank accession no. AF196835.

Main Article

Page created: July 19, 2010

Page updated: July 19, 2010

Page reviewed: July 19, 2010

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

Volume 8, Number 12—December 2002

Research

First Isolation of West Nile virus from a Patient with Encephalitis in the United States

Table 1