Volume 9, Number 12—December 2003
Research
Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis
Isabelle Podglajen*†, Fabienne Bellery*, Claire Poyart†, Philippe Coudol*, Annie Buu-Hoï*, Patrick Bruneval*, and Jean-Luc Mainardi*†
Table
Comparison of results obtained from blood cultures with conventional methods of identification (CMI-BC) and from valves with sequence-based identification (SBI-V)
| Positive blood culture (n = 30) | Negative blood culture (n = 6) | |||||
|---|---|---|---|---|---|---|
| PCR positive (n = 26) |
PCR negative (n = 4)a |
PCR positive (n = 5) |
PCR negative
n = 1 |
|||
| Species identification |
||||||
| Concordance
(n = 16) |
Discordance (n = 10) |
|||||
| (CMI-BC/SBI-V) |
CMI-BC |
SBI-V |
CMI-BC |
SBI-V |
||
| Streptococcus mutans S. sanguis S. gallolyticus (4) Campylobacter fetus Escherichia colib Propionibacterium acnes Staphylococcus aureus (2) Streptococcus oralis (2)c S. mitis (2)c S. pneumoniaec | S. agalactiae Gemella spp. Group C streptococci S. mitis (2) S. sanguis S. salivarius Haemophilus influenzae H. aphrophilus Streptococcus spp. | Lactobacillus crispatus Aerococcus urinae Abiotrophia adiacens S. sanguis (2) S. oralisc S. oralisc H. aphrophilus H. paraphrophilus S. gordonii | Staphylococcus aureus
S. epidermidis
S. caprae
S. aureus +
Strep. pneumonia 
+ E. coli |
S. gallolyticus Bartonella quintana (3) B. henselae d | ||
aIn the case of positive blood cultures with Staphylococcus aureus, Escherichia coli, and Streptococcus (Strep) pneumoniae, PCR was positive, but direct sequencing was not interpretable.
bSpecies identification based on sequence analysis of PCR-amplified rpoBint
cSpecies identification based on sequence analysis of PCR-amplified sodAint
dThree species were identified in the blood culture.
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