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Volume 16, Number 2—February 2010
Dispatch

White-Nose Syndrome Fungus (Geomyces destructans) in Bat, France

Sébastien J. Puechmaille, Pascal Verdeyroux, Hubert Fuller, Meriadeg Ar Gouilh, Michaël Bekaert, and Emma C. TeelingComments to Author 
Author affiliations: University College Dublin, Dublin, Ireland (S.J. Puechmaille, H. Fuller, M. Bekaert, E.C. Teeling); Groupe Chiroptères Aquitaine, Erdoia, France (P. Verdeyroux); Institut Pasteur, Paris, France (M. Ar Gouilh)

Main Article

Table

Primers used for PCR amplification and sequencing of fungus in bats, France*

Gene Primer sequence (5′ → 3′) PCR
ITS
 TCCTCCGCTTATTGATATGC Forward
GGAAGTAAAAGTCGTAACAAGG
 Reverse
SSU rRNA CTGGTTGATTCTGCCAGT Forward
AAACCTTGTTACGACTTTTA Reverse
CCGGAGAAGGAGCCTGAGAAAC †
AACTTAAAGGAATTGACGGAAG †
CTCATTCCAATTACAAGACC †
GAGTTTCCCCGTGTTGAGTC †

*ITS, internal transcribed spacer; SSU, small subunit.
†Used for sequencing only.

Main Article

Page created: December 13, 2010
Page updated: December 13, 2010
Page reviewed: December 13, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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