Volume 15, Number 4—April 2009
Dispatch
Genetic Diversity of Toscana Virus
Table
Comparison | Similarity of sequences (amino acid similarity), mean % ± SD |
|||
---|---|---|---|---|
L segment | M segment | NS gene | N gene | |
Within TOSV | 91.7 ± 0.7 (98.8 ± 0.5) | 92.4 ± 0.6 (95.4 ± 0.9) | 90.7 ± 0.8 (95.8 ± 1) | 95.9 ± 0.8 (100) |
Within TOSV A | NA | 99.3 ± 0.1 (99.4 ± 0.5) | NA | 100 (100) |
Within TOSV B | 98.2 ± 0.2 (99.7 ± 0.1) | 95.5 ± 0.4 (98.2 ± 0.5) | 97.3 ± 0.4 (98.5 ± 0.5) | 98.5 ± 0.3 (100) |
Between TOSV A and TOSV B | 85.2 ±0.4 (97.0 ± 0.4) | 81.8 ± 0.4 (90.0 ± 1.1) | 83.6 ± 1.1 (89.6 ± 1.6) | 87.9 ± 1.4 (100) |
Within TOSV A G1 to G4 | NA | 99.3 ± 0.1 (99.3 ± 0.2) | NA | NA |
Between TOSV A G1, G2, G3, and G4 | NA | 95.6 ± 0.4 (97.3 ± 0.5) | NA | NA |
Within RVFV | 97.7 ± 0.3 (99.8 ± 0.1) | 95.4 ± 0.5 (98.6 ± 0.5) | 97.2 ± 0.3 (98.5 ± 0.4) | 97.6 ± 0.5 (99.8 ± 0.1) |
Within RVFV lineages | 98.8 ± 0.1 (99.5 ± 0.1) | 98.8 ± 0.1 (99.5 ± 0.1) | 98.8 ± 0.2 (99.1 ± 0.3) | 99.1 ± 0.2 (99.9 ± 0.1) |
Between RVFV lineages | 96.6 ± 0.2 (99.2 ± 0.2) | 96.6 ± 0.2 (99.1 ± 0.2) | 97.0 ± 0.5 (98.0 ± 0.6) | 97.5 ± 0.5 (99.6 ± 0.3) |
Within SFSV | NA | 76.5 ± 1.3 (80.2 ± 2.1) | 99.7 ± 0.2 (98.9 ± 0.7) | 100 (100) |
Within SFNV | NA | 72.2 ± 1.2 (72.9 ± 2.1) | NA | NA |
Within PTV | 96.5 ± 0.7 (100) | 92.5 ± 0.8 (97.5 ± 0.9) | 87.6 ± 1.4 (94.2 ± 1.6) | 100 (100) |
Between SFSV and PTV | NA | 45.5 ± 1.8 (32.3 ± 3.2) | 40.2 ± 1.9 (23.7 ± 3.1) | 55.0 ± 3.6 (52.5 ± 6.4) |
Between SFNV and TOSV | NA | 57.2 ± 1.7 (53.6 ± 3.1) | NA | 78.8 ± 2.8 (93.2 ± 3.3) |
*Based on complete genome sequences. To estimate the evolutionary divergence over sequence pairs between groups, the average number of nucleotide differences per site over all sequence pairs between groups and among groups was calculated. Standard error estimates were obtained by a bootstrap procedure (500 replicates). For each pairwise sequence comparison, all positions containing alignment gaps and missing data were eliminated. L, large; M, medium; NS, nonstructural; N, nucleoprotein; TOSV, Toscana virus; NA, not applicable; RVFV, Rift Valley fever virus; SFSV, sandfly fever Sicilian virus; SFNV, sandfly fever Naples virus; PTV, Punta Toro virus.
1,3These pairs of authors contributed equally to this work
2Current affiliation: Valparaíso University, Valparaíso, Chile