The Tropical Medicine Foundation of Amazonas is a tertiary care center for infectious diseases in Manaus (3°8′S, 60°1′W). In 2001 and 2002, a total of 13,056 cases of malaria were diagnosed in this institution (11,251 P. vivax), representing 65.1% of the total cases from Manaus. During the same period, 358 (3.2%) patients with P. vivax malaria were hospitalized. A retrospective analysis was performed of case-patients who fulfilled the malaria severity criteria of the World Health Organization (WHO) (8). These patients had an exclusive diagnosis of P. vivax malaria by thick blood smear (reviewed 2 times by experienced microscopists) and PCR, according to the technique described elsewhere (9).

PCR was performed on whole blood from all patients with P. vivax malaria, confirmed by microscopy and any P. falciparum severity criterion recommended by WHO. Blood specimens were routinely stored by the laboratory of the institution at –70°C. Full clinical information was available from the patients’ charts, and serologic tests for dengue virus, Leptospira spp., and hepatitis A, B, and C viruses were performed on available serum samples stored at –20°C.

Each patient was monitored for 28 days in outpatient clinics after beginning antimalarial treatment. Patients were routinely discharged only after parasitologic clearance and clinical recovery. Until 2006, chloroquine was still prescribed for patients with severe cases at a dose of 10 mg/kg on the first day and 7.5 mg/kg on the second and third days, followed by primaquine (0.5 mg/kg/day for 7 days), according to the Brazilian Ministry of Health guidelines. In 2006, WHO formally recommended the treatment of severe vivax malaria to be the same as that for severe falciparum malaria, because of the risk for an unrecognized mixed infection (8).

Seventeen patients were included in the analysis, and their clinical and laboratory data are shown in Table 1 and Table 2, respectively. All patients received chloroquine (orally or through a nasogastric tube) and primaquine. Acute respiratory distress syndrome (ARDS) (diffuse interstitial and alveolar infiltrate by chest radiograph and partial O₂ pressure 40 mm Hg by arterial gas analysis) developed in patient 11 two days after she received chloroquine, and she died 3 days later. This patient had a negative thick blood smear from day 3 of treatment with choloroquine. The other 16 patients were followed up after discharge until day 28. None had clinical symptoms of malaria, and all thick blood smears were negative at days 7, 14, and 28.

Figure

Figure. Hand of a 2-year-old child (patient no. 1) with severe anemia (hemoglobin level 3.6 g/dL), showing intense pallor, compared with the hand of a healthy physician. Photograph provided by authors.

The patients in whom complications developed exhibited a remarkably wide age range (28 days–80 years). This age range is similar to that seen in other case series from Latin America, such as in hospitalized children from Venezuela with severe anemia that required blood transfusions (10) and in adults from Rondônia (a state in the western Brazilian Amazon) who had severe anemia, jaundice, acute renal failure, ARDS, and shock (11). P. vivax malaria with ARDS has been reported in travelers who acquired the infection in Manaus (12,13). The finding of severe anemia in 4 of 7 children highlights the relevance of this complication in P. vivax infection (Figure), as shown in a prospective study from Papua, Indonesia (14). Nine patients sought treatment for cholestatic jaundice; for 8, jaundice was the only complication. The mechanisms involved are unknown.

The concomitant diagnosis of hepatitis A virus infection in patient 3 (Table 1) indicates that other infectious diseases should be excluded when characterizing severe P. vivax malaria. Also of note is the presence of thrombocytopenia in 15/17 patients (none had clinical bleeding), which suggests that this hematologic complication may be a surrogate marker of severity.

The wide range of parasitemia found in our patients does not enable us to comment on the value of this variable as a determinant of severity. Sixteen patients recovered without the use of antimicrobial drugs; therefore, it is highly improbable that bacterial sepsis was a factor for severity in our case series. In unstable transmission areas (<0.1 autochthonous case per 1,000 persons per year), malaria in older patients may pose an additional problem because chronic diseases (e.g., arterial hypertension and diabetes) may predispose a patient to clinical decompensation.

In vivo chloroquine resistance was not detected in any of the cases that were followed up, despite recent confirmation of the phenomenon in this same locality (15). Because a reliable molecular marker of chloroquine resistance is lacking and parenteral artemisinin derivatives are recommended for treatment of patients with severe P. vivax malaria, studies that assess clinical severity and chloroquine resistance would be unethical. However, our findings suggest that chloroquine resistance would be a problem for individual patients and that the determinants of this resistance need to be clarified. Clearly, areas with chloroquine-resistant P. vivax also report severe P. vivax malaria, but we believe that these studies are not able to establish any firm causality. The finding of both phenomena in some areas may simply reflect high transmission of this species.

Our retrospective review illustrates the spectrum of severe P. vivax malaria in Manaus, and these results parallel the increasing clinical severity described in malaria-endemic areas such as Papua (Indonesia) and India. These severe P. vivax cases contribute to increased public health costs because of increased hospitalization and the need for intensive care and blood transfusions. The major complications in patients who required hospitalization were jaundice and severe anemia, although whether these complications were responsible for deaths is undetermined.

No clear severity criteria exist for P. vivax malaria. However, WHO criteria formerly defined for P. falciparum malaria seem to be applicable to most of the severe P. vivax malaria cases reported in hospital-based studies in the literature. PCR should be performed to rule out mixed infections and other common infectious diseases so that reports from different parts of the world are comparable. Despite the small number of patients, our data corroborate previous findings of severe disease found in areas where chloroquine-resistant P. vivax is being reported but suggest that establishing direct causality is not straightforward. We urgently need to know which clinical complications in P. vivax malaria are associated with death to validate severity criteria. A valid biomarker for chloroquine resistance would also enable associative studies to determine the association between resistance and severity.