Volume 20, Number 1—January 2014
Dispatch
Avian Hepatitis E Virus in Chickens, Taiwan, 2013
Ingrid W.-Y. Hsu and Hsiang-Jung Tsai
Table 2
Primers used in the generation of the complete genome of the Taiwan avian hepatitis E virus strain*
| Primer name† | Primer sequence, 5′→3′ | Direction |
|---|---|---|
| TWN6490–6470 | CTAGAAGTCGGCGTGTCTCAG | R |
| TWN6469–6448 | GTGACTGGTCCTCAGGTGCTTG | R |
| TWN6244–6270 | CAGGAGTGGATCTATTTCCTTCAGAAC | F |
| TWN6243–6262 | CCAGGAGTGGATCTATTTCC | F |
| TWN6215–6241 | GATACTTCTATCAGTACAACAACACAC | F |
| TWN5705–5686 | CGCAGCAGCGTGGATGGTAG | R |
| TWN5506–5526 | GTTAAGGTGACTGCTCCGCAC | F |
| TWN5043–5023 | GTCCTGAAGTGGCATGAGCGG | R |
| TWN4784–4763 | CTTCCGGCTGGGAGCGTTTGGG | R |
| TWN3421–3441 | CCATTGTCGCCTGGCTGCACC | F |
| TWN3227–3247 | GACGGGTTATTGGATATACCG | F |
| TWN2952–2932 | GGGTTCGACTGCTTGGCCACC | R |
| TWN2930–2909 | GAGTAAACACAATTTTTTGGCC | R |
| TWN2569–2588 | CGGGGCTGTTGCGATTACGC | F |
| TWN1657–1635 | CATAATGTGCAACGATGGCGGCG | R |
| TWN1607–1582 | CAGCAAGCTCTTTAAGTGTGAGTAGC | R |
| TWN1570–1546 | GAGGTCAATCAAATTCTCAGTGCTG | R |
| TWN1145–1123 | CAGCAATGGCAACAGCCGTCAGC | R |
| TWN1114–1091 | CTCAGGCTGCCAACCCTCATTGGC | R |
| TWN1048–1026 | GTAGGTCAGCAAGCGCGAGCAGC | R |
| TWN670–646 | CTTGTCCGTTGTATTTACGGTATTG | R |
| TWN607–584 | CTCCTCTGGTAAGTGCAACACGAC | R |
| TWN580–557 | CAGTGTCCGCATATTATGGCGGGC | R |
| HaHEV1–26‡ | GCGGCCGCTCTAGCTGCAGCGAATAC | F |
| HaHEV1410–1433‡ | CATCCGTGCGGGTACTAAATCTGC | F |
| Anchored-oligo (dT)18 primer | NVTTTTTTTTTTTTTTTTTT | NA |
| Oligo d(T)-anchor primer | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV | NA |
*The annealing temperature was set at 60°C for each primer combination. R, reverse; F, forward; NA, not applicable.
†Numbers following TWN in primer names is the nucleotide position extracted by the respective primers.
‡Primers were designed on the basis of the sequence from Hungary (GenBank accession no. JN997392).
Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
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