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Volume 21, Number 1—January 2015
Research

Protocol for Metagenomic Virus Detection in Clinical Specimens1

Claudia KohlComments to Author , Annika Brinkmann, Piotr W. Dabrowski, Aleksandar Radonić, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Table 2

Sample preparation, infection dose, and copy numbers for virus-infected embryonated chickens after 7 d used to develop a protocol for metagenomic virus detection in infectious disease settings*

Virus family Strain Infection dose Virus-positive organ Virus copy number/100 µL*
Reovirinae T3/Germany/Bat/342/08 10−3 Liver 3.47 × 103
Orthomyxovirinae Influenza A/PR/8/1932 10−6 Lung 4.94 × 104
10−7 Lung 8.32 × 102
10−5 Liver 8.74 × 104
10−4 Liver 1.66 × 102
Poxvirinae Vaccinia virus 10−2 Kidney 1.83 × 108
10−2 Kidney 2.37 × 108
10−2 Kidney 2.38 × 108
10−2 Intestine 2.23 × 108
10−1 Liver 1.92 × 108
10−2 Liver 9.00 × 107
10−2 Kidney 2.35 × 108
Paramyxovirinae Sendai virus 10−2 Liver 2.13 × 103
10−2 Liver 2.14 × 105
10−3 Liver 1.86 × 105

*Homogenized tissue with an average volume of 8 mm3/ mL.

Main Article

1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.

Page created: January 05, 2015
Page updated: January 05, 2015
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