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Volume 26, Number 6—June 2020
Research

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Jennifer Harcourt1, Azaibi Tamin1, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery2, and Natalie J. Thornburg2Comments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Harcourt, A. Tamin, X. Lu, K. Queen, Y. Tao, C.R. Paden, Y. Li, C. Goldsmith, B. Whitaker, R. Gautam, S. Lindstrom, S. Tong, N.J. Thornburg); Eagle Medical Services, Atlanta (S. Kamili, S.K. Sakthivel, J. Murray, B. Lynch); IHRC, Atlanta (J. Zhang, H. Wang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara); Synergy America, Inc., Atlanta (H.A. Bullock, L. Wang); University of Texas Medical Branch, Galveston, Texas, USA (C. Schindewolf, K.G. Lokugamage, D. Mirchandani, S. Widen, K. Narayanan, S. Makino, T.G. Ksiazek, S.C. Weaver, V.D. Menachery); World Reference Center for Emerging Viruses and Arboviruses, Galveston (D. Scharton, J.A. Plante, T.G. Ksiazek, K.S. Plante, S.C. Weaver, V.D. Menachery)

Main Article

Figure 2

Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified by using plaque assay at day 2 (solid circles) and day 3 (open circles) postinfection of Vero E6 and Vero CCL81 cells. B) Plaque morphology for virus on Vero E6 and Vero CCL81 at day 2 and day 3 postinoculation. C) Cell monolayers 2 days postinfection of Vero E6 (top) and

Figure 2. Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified by using plaque assay at day 2 (solid circles) and day 3 (open circles) postinfection of Vero E6 and Vero CCL81 cells. B) Plaque morphology for virus on Vero E6 and Vero CCL81 at day 2 and day 3 postinoculation. C) Cell monolayers 2 days postinfection of Vero E6 (top) and Vero CCL81 (bottom) at 3 dilutions. Original magnifications ×40.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: May 18, 2020
Page updated: May 18, 2020
Page reviewed: May 18, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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