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Volume 26, Number 6—June 2020
Research

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Jennifer Harcourt1, Azaibi Tamin1, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery2, and Natalie J. Thornburg2Comments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Harcourt, A. Tamin, X. Lu, K. Queen, Y. Tao, C.R. Paden, Y. Li, C. Goldsmith, B. Whitaker, R. Gautam, S. Lindstrom, S. Tong, N.J. Thornburg); Eagle Medical Services, Atlanta (S. Kamili, S.K. Sakthivel, J. Murray, B. Lynch); IHRC, Atlanta (J. Zhang, H. Wang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara); Synergy America, Inc., Atlanta (H.A. Bullock, L. Wang); University of Texas Medical Branch, Galveston, Texas, USA (C. Schindewolf, K.G. Lokugamage, D. Mirchandani, S. Widen, K. Narayanan, S. Makino, T.G. Ksiazek, S.C. Weaver, V.D. Menachery); World Reference Center for Emerging Viruses and Arboviruses, Galveston (D. Scharton, J.A. Plante, T.G. Ksiazek, K.S. Plante, S.C. Weaver, V.D. Menachery)

Main Article

Figure 3

Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and subsequently harvested 24 h postinfection for viral titer and protein lysates. A) Viral titer for SARS-CoV-2 quantitated by plaque assay on Vero E6 cells 2 days postinoculation. Infected cell protein lysates were probed by using Western blotting with B) rabbit polyclonal anti-SAR

Figure 3. Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and subsequently harvested 24 h postinfection for viral titer and protein lysates. A) Viral titer for SARS-CoV-2 quantitated by plaque assay on Vero E6 cells 2 days postinoculation. Infected cell protein lysates were probed by using Western blotting with B) rabbit polyclonal anti-SARS N antibody or C) anti–SARS-CoV S protein antibody. Full-length spike protein (SFL) and spike protein S1 (S1) are indicated. N, nucleocapsid; S, spike protein; SARS, severe acute respiratory syndrome.

Main Article

1These authors contributed equally to this article.

2These senior authors contributed equally to this article.

Page created: May 18, 2020
Page updated: May 18, 2020
Page reviewed: May 18, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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