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Volume 30, Number 1—January 2024
Dispatch

Avian Influenza A(H5N1) Neuraminidase Inhibition Antibodies in Healthy Adults after Exposure to Influenza A(H1N1)pdm09

Pavithra Daulagala, Samuel M.S. Cheng, Alex Chin, Leo L.H. Luk, Kathy Leung, Joseph T. Wu, Leo L.M. Poon, Malik Peiris1Comments to Author , and Hui-Ling Yen1Comments to Author 
Author affiliations: School of Public Health, The University of Hong Kong, Hong Kong, China (P. Daulagala, S.M.S. Cheng, A. Chin, L.H.L. Luk, K. Leung, J.T. Wu, L.L.M. Poon, M. Peiris, H.L. Yen); The University of Hong Kong–Shenzhen Hospital, Shenzhen, China (K. Leung); Centre for Immunology and Infection, Hong Kong Science Park, Hong Kong (L.L.M. Poon, M. Peiris)

Main Article

Table 1

NAI antibody titers detected in postinfection ferret antiserum against influenza A(H1N1), A(H1N1)pdm09, and A(H5N1) viruses, Hong Kong, China

Postinfection
ferret antiserum†		 NAI antibody titers*
Homologous virus California/09 A (H1N1)pdm09 Spoonbill/HK/22 A(H5N1)
A/USSR/90/1977 320 <10 <10
A/Chile/01/1983 320 <10 <10
A/Singapore/06/1986 320 <10 <10
A/Texas/36/1991 320 <10 <10
A/Brisbane/59/2007 1,280 <10 <10
A/California/04/2009 2,560 2,560 320–640

*NAI antibody response was determined using recombinant A(H6N1) viruses carrying the N1 proteins derived from A(H1N1), A(H1N1)pdm09, or A(H5N1) viruses that were generated as described previously (10). Although we used recombinant H6N1 viruses in ELLA assays to avoid interference of hemagglutinin-reactive antibodies, we might not completely prevent the interference of cross-reactive antibodies that bind to the stalk of H6 protein and inhibit neuraminidase activity through stearic hindrance. NAI, neuraminidase inhibition. †One ferret antiserum against each of the influenza A(H1N1) viruses and 6 ferret antiserum against the influenza A(H1N1)pdm09 were used.

Main Article

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1These senior authors contributed equally to this article.

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