Table 3

Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes

Cristina Rafferty, Gloria Raise, JeNyiah Scaife, Bernard Abongo, Seline Omondi, Sylvia Milanoi, Margaret Muchoki, Brenda Onyango, Eric Ochomo, and Sarah Zohdy

Author affiliations: US President’s Malaria Initiative, Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Rafferty, S. Zohdy); Centers for Disease Control and Prevention, Atlanta (C. Rafferty, G. Raise, J. Scaife, S. Zohdy); Kenya Medical Research Institute (KEMRI), Kisumu, Kenya (B. Abongo, S. Omondi, S. Milanoi, M. Muchoki, B. Onyango, E. Ochomo)

Main Article

Primer set candidates tested with different concentrations to determine effects on sensitivity and specificity of colorimetric loop-mediated isothermal amplification assay to detect invasive malaria vector Anopheles stephensi mosquitoes

Concentration combination						Primer set*
	Primer concentration					P26L2		P2L-45
	F3	B3	FIP	B IP	Loop primer	Sensitivity	Specificity	Sensitivity	Specificity
A1	2 µM	2 µM	16 µM	16 µM	4 µM	X	✓	X	X
A2	4 µM	4 µM	16 µM	16 µM	4 µM	✓	X	X	X
A3	2 µM	2 µM	32 µM	32 µM	4 µM	✓	X	X	X
A4	2 µM	2 µM	16 µM	16 µM	8 µM	X	✓	X	X
B1	5 µM	5 µM	40 µM	40 µM	10 µM	✓	X	✓	X
B2	2.5 µM	2.5 µM	40 µM	40 µM	10 µM	✓	X	X	✓
B3	5 µM	5 µM	20 µM	20 µM	10 µM	✓	✓	X	X
B4	5 µM	5 µM	40 µM	40 µM	5 µM	✓	X	X	X

*For each primer set, a check mark indicates consistent results and an X inconsistent results.

Main Article

Page created: July 10, 2024

Page updated: August 20, 2024

Page reviewed: August 20, 2024

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