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Volume 31, Number 5—May 2025
Research

Detection of SARS-CoV-2 Reinfections Using Nucleocapsid Antibody Boosting

Eduard GrebeComments to Author , Daniel Chacreton, Mars Stone, Bryan R. Spencer, James Haynes, Akintunde Akinseye, Marion C. Lanteri, Valerie Green, Hasan Sulaeman, Roberta Bruhn, Vivian I. Avelino-Silva, Paul Contestable, Brad J. Biggerstaff, Melissa M. Coughlin, Brian Custer, Jefferson M. Jones, David Wright1, and Michael P. Busch1
Author affiliation: Vitalant Research Institute, San Francisco, California, USA (E. Grebe, M. Stone, H. Sulaeman, R. Bruhn, V.I. Avelino-Silva, B. Custer, M.P. Busch); Westat, Rockville, Maryland, USA (D. Chacreton, A. Akinseye, D. Wright); University of California San Francisco, San Francisco (M. Stone, R. Bruhn, B. Custer, M.P. Busch); American Red Cross, Dedham, Massachusetts, USA (B.R. Spencer); American Red Cross, Rockville (J.M. Haynes); Creative Testing Solutions, Tempe, Arizona, USA (M.C. Lanteri, V. Green); San Francisco State University, San Francisco (H. Sulaeman); QuidelOrtho, Rochester, New York, USA (P. Contestable); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (B.J. Biggerstaff); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.M. Coughlin, J.M. Jones)

Main Article

Table 2

Reinfection classification data determined from ratio approach in study of SARS-CoV-2 reinfections detected by nucleocapsid antibody boosting*

Cases
 Controls
 Unweighted Youden's J (1.43)
 Weighted Youden's J (2.33)
S/CO No. Median ratio No. Median ratio Sensitivity, % Specificity, % Sensitivity, % Specificity, %
Overall 2,681 6.22 5,150 0.55 87.1 96.0 75.3 99.3
0–50 1,661 15.2 2,237 0.49 93.8 97.0 91.6 98.9
50–100 485 3.41 1,042 0.49 89.5 97.3 81.9 99.2
100–150 275 2.15 757 0.56 88.0 91.7 35.6 99.9
>150 260 1.28 1,114 0.76 38.8 95.5 0.8 100.0

*S/CO value is prereinfection. Unweighted and weighted boosting thresholds are ratios of postreinfection to prereinfection S/CO. S/CO, signal-to-cutoff ratio.

Main Article

1These senior authors contributed equally to this article.

Page created: March 25, 2025
Page updated: April 22, 2025
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