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Volume 26, Number 2—February 2020
Research

Interspecies Transmission of Reassortant Swine Influenza A Virus Containing Genes from Swine Influenza A(H1N1)pdm09 and A(H1N2) Viruses

Helen E. EverettComments to Author , Bethany Nash, Brandon Z. Londt1, Michael D. Kelly, Vivien Coward, Alejandro Nunez, Pauline M. van Diemen, Ian H. Brown, and Sharon M. Brookes
Author affiliations: Animal and Plant Health Agency, Weybridge, UK

Main Article

Figure 1

Infection and replication of swine H1N2r virus in ferret tracheal organ cultures. A) Quantity of influenza A virus RNA in ferret lung ex vivo organ cultures at 24 h and 48 h postinoculation with swine viruses H1N2r (A/swine/England/1382/2010), Sw/pdm09 (A/swine/England/1353/2009), H1N2 (A/swine/England/997/2009), and Hu/pdm09 (A/England/195/2009). Results are log10 REU in combined supernatants and tissue lysates for each sample. Error bars indicate upper end of SEM for triplicate cultures. B) Im

Figure 1. Infection and replication of swine H1N2r virus in ferret tracheal organ cultures. A) Quantity of influenza A virus RNA in ferret tracheal ex vivo organ cultures at 24 h and 48 h postinoculation with swine viruses H1N2r (A/swine/England/1382/2010), Sw/pdm09 (A/swine/England/1353/2009), H1N2 (A/swine/England/997/2009), and Hu/pdm09 (A/England/195/2009). Results are log10 REU in combined supernatants and tissue lysates for each sample. Error bars indicate upper end of SEM for triplicate cultures. B) Immunohistochemical labeling of influenza A virus nucleoprotein in ferret tracheal cultures infected with H1N2r reassortant virus (original magnification ×20). C) Detection of virus-positive strand replicative intermediate RNA in ferret tracheal and lung cultures at 24 and 48 h postinoculation with H1N2r virus. Error bars indicate upper end of SEM for triplicate cultures. Ct, cycle threshold; hpi, hours postinoculation; hu, human; H1N2r, reassortant swine influenza A virus; pdm09, influenza A(H1N1)pdm09; REU, relative equivalent unit; sw, swine; +, positive.

Main Article

1Current affiliation: hVIVO Services Limited, London, UK.

Page created: January 17, 2020
Page updated: January 17, 2020
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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