Figure 3. Nasal shedding and transmission of swine influenza A(H1N2) reassortant virus from infected animals. A) Schematic outline of study design. B, C) In study I, virus RNA in nasal swab specimens from directly infected or contact pigs (B) and directly infected or contact ferrets (C) was quantified by using real-time reverse transcription quantitative PCR. Values are indicated as REU at each dpi or dpc. Error bars indicate SEM. D) AUC for virus shedding profiles in study I in pigs or ferrets, showing significant differences (****p<0.0001) in virus shedding by directly infected ferrets compared with all other groups and between contact pigs and ferrets. Error bars indicate upper end of SEM. E, F) In study II, virus RNA in nasal swab specimens from infected or cohoused pigs (E) or nasal washes from infected or cohoused ferrets (F) was quantified as REU. Error bars indicate SEM. AUC, area under the curve; dpi, days postinfection; dpc, day postcontact; REU, relative equivalent unit.